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|Antigen||SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies|
|Epitope||AA 359-473 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SHC1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
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Product Details anti-SHC1 AntibodyTarget Details SHC1 Application Details Handling References for anti-SHC1 antibody (ABIN968229) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human SHC aa. 359-473|
Target Details SHC1Product Details anti-SHC1 Antibody Application Details Handling References for anti-SHC1 antibody (ABIN968229) Images back to top
|Alternative Name||SHC (SHC1 Antibody Abstract)|
|Background||The mammalian SHC proteins, which are expressed as multiple isoforms (46, 52, and 66 kDa), each contain a C-terminal SH2 domain and an N-terminal glycine/proline rich sequence. These proteins function as early signaling intermediates (also called adaptor proteins) which relay G protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK)-induced signals via the Ras transduction pathway. To this end, the SHC proteins contain specific tyrosine residues which are phosphorylated following association with the active RTKs. Phosphorylated SHC forms a complex with the adaptor protein GRB2. Association of the SHC-GRB2 complex with the Ras guanine nucleotide exchange factor (Ras-GEF) mediates the localization of Ras-GEF to the plasma membrane. Once at the plasma membrane, Ras-GEF activates Ras by catalyzing the Ras-GTP for Ras-GDP exchange. Over-expression of SHC results in cell transformation, and phosphorylation of SHC correlates with activation of the ERK1/ERK2 kinases. The SHC proteins are mediators of signals that are essential for cell metabolism, growth, and differentiation.|
|Molecular Weight||66, 52 & 46 kDa|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-SHC1 Antibody Target Details SHC1 Handling References for anti-SHC1 antibody (ABIN968229) Images back to top
Related Products: ABIN968535, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details References for anti-SHC1 antibody (ABIN968229) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-SHC1 antibody (ABIN968229)Product Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling Images back to top
|Product cited in:||
Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, 2002
Ugi, Imamura, Ricketts, Olefsky: "Protein phosphatase 2A forms a molecular complex with Shc and regulates Shc tyrosine phosphorylation and downstream mitogenic signaling." in: Molecular and cellular biology, Vol. 22, Issue 7, pp. 2375-87, 2002
Imokawa, Kobayasi, Miyagishi: "Intracellular signaling mechanisms leading to synergistic effects of endothelin-1 and stem cell factor on proliferation of cultured human melanocytes. Cross-talk via trans-activation of the tyrosine kinase c-kit receptor." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33321-8, 2000
Laser, Willey, Jiang, Cooper, Menick, Zile, Kuppuswamy: "Integrin activation and focal complex formation in cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35624-30, 2000
Pelicci, Lanfrancone, Grignani, McGlade, Cavallo, Forni, Nicoletti, Grignani, Pawson, Pelicci: "A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction." in: Cell, Vol. 70, Issue 1, pp. 93-104, 1992
ImagesProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling References for anti-SHC1 antibody (ABIN968229) back to top