anti-Human TEK antibody for Intracellular Staining

Recommended TEK Antibody (supplied by: Log in to see )

Antigen
TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
Epitope
pTyr1102
29
28
23
23
23
19
17
15
14
14
14
11
10
7
6
6
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
365
178
85
9
7
Host
Mouse
204
168
48
3
3
2
Clonality
Monoclonal
Conjugate
This TEK antibody is conjugated to Alexa Fluor 647
29
21
19
16
14
14
13
9
8
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
2
Application
Intracellular Staining (ICS)
266
194
140
136
63
53
43
33
22
12
8
7
5
5
4
4
3
2
1
Options
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Catalog No. ABIN1177219
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177218 ICS PE Mouse IgG2a, kappa pTyr1102 Log in to see K93-754 6
1 ABIN1177217 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr992 Log in to see K91-860 6
1 ABIN1177220 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr1102 Log in to see K93-754 6

General

Antigen TEK Tyrosine Kinase, Endothelial (TEK) Antibodies
Epitope pTyr1102
(29), (28), (23), (23), (23), (19), (17), (15), (14), (14), (14), (11), (10), (7), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(365), (178), (85), (9), (7)
Host Mouse
(204), (168), (48), (3), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TEK antibody is conjugated to Alexa Fluor 647
(29), (21), (19), (16), (14), (14), (13), (9), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2)
Application Intracellular Staining (ICS)
(266), (194), (140), (136), (63), (53), (43), (33), (22), (12), (8), (7), (5), (5), (4), (4), (3), (2), (1)
Pubmed 6 references available
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Product Details anti-TEK Antibody

Target Details TEK Application Details Handling References for anti-TEK antibody (ABIN1177219) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human TIE2 Peptide
Clone K93-754
Isotype IgG2a, kappa

Target Details TEK

Product Details anti-TEK Antibody Application Details Handling References for anti-TEK antibody (ABIN1177219) Images back to top
Antigen
Alternative Name TIE2 (TEK Antibody Abstract)
Background TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. Phosphorylation at tyrosine 1102 (Y1102) leads to activation of the Akt signal transduction pathway, enhancing endothelial cell survival. The phosphorylated Y1102 also recruits and activates the src homology 2 domain-containing adaptor proteins Grb2 and ShcA, affecting endothelial cell adhesion and motility. The K93-754 monoclonal antibody recognizes the phosphorylated Y1102 in the C-terminal tail of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y1100.
Synonyms: TEK, VMCM, CD202b
Research Area Tyrosine Kinases
Pathways RTK Signaling, Growth Factor Binding

Application Details

Product Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK antibody (ABIN1177219) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK antibody (ABIN1177219) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TEK antibody (ABIN1177219)

Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
Product cited in:

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, 2004

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, 2004

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, 2004

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, 2001

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, 2001

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, 1983