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|Antigen||TEK Tyrosine Kinase, Endothelial (TEK) Antibodies|
|Epitope||AA 450-500 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TEK antibody is un-conjugated Alternatives|
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-TEK AntibodyTarget Details TEK Application Details Handling References for anti-TEK antibody (ABIN674649) Images
|Purification||Purified by Protein A.|
|Immunogen||KLH conjugated synthetic peptide derived from human Tie2|
Target Details TEKProduct Details anti-TEK Antibody Application Details Handling References for anti-TEK antibody (ABIN674649) Images back to top
|Alternative Name||Tie2/Cd202b (TEK Antibody Abstract)|
Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.
Subcellular location: Extracellular
Synonyms: TIE2, VMCM, TIE-2, VMCM1, CD22B, Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p14 TEK, TEK
|Pathways||RTK Signaling, Growth Factor Binding|
Application DetailsProduct Details anti-TEK Antibody Target Details TEK Handling References for anti-TEK antibody (ABIN674649) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-TEK Antibody Target Details TEK Application Details References for anti-TEK antibody (ABIN674649) Images back to top
|Buffer||Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Storage Comment||Store at -20°C|
|Expiry Date||12 months|
References for anti-TEK antibody (ABIN674649)Product Details anti-TEK Antibody Target Details TEK Application Details Handling Images back to top
|Product cited in:||
Li, Liu, Liu, Wang, Zhang, Chen, Hui: "Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation." in: Neuroscience letters, Vol. 639, pp. 120-125, 2017 Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).
Xiang, Hu, Shen, Liu, Hua, Zan, Zu, Cui, Ye: "Bone marrow mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke in type 2 diabetic rats." in: Neuroscience letters, Vol. 644, pp. 62-66, 2017 Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).
Tekari, Chan, Sakai, Grad, Gantenbein: "Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells." in: Stem cell research & therapy, Vol. 7, Issue 1, pp. 75, 2016
Zhao, Hu, Xiang, Gu, Jin, Hua, Zhang, Liu, Zan, Zhang, Zu, Yang, Shi, Zhu, Xu, Cui, Ye: "Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke." in: Brain research, 2015
ImagesProduct Details anti-TEK Antibody Target Details TEK Application Details Handling References for anti-TEK antibody (ABIN674649) back to top