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|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
|1 reference available|
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Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN181548) Images
|Specificity||This antibody detects VEGF.|
|Immunogen||Highly pure (> 98 %) recombinant human VEGF.|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN181548) Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
|Background||Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN181548) Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman VEGF (10 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody is required. Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL is required. In conjunction with BiotinylatedAnti-Human VEGF (ABIN181546 or ABIN181545) as a Detection antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human VEGF. Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human VEGF is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry: 5.0-15.0 μg/mL with 30 minutes at RT or an overnight incubation at4 °C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABchromogen. This antibody was also tested in an HRP-Biotin labeling detection system. Heat induced antigen retrieval with pH 6.0 Sodium Citrate buffer is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN181548) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.|
|Expiry Date||6 months|
References for anti-VEGF antibody (ABIN181548)Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Hasson, Gallula, Shimoni, Grad-Itach, Marikovsky, Mitrani: "Skin-derived micro-organs induce angiogenesis in rabbits." in: Journal of vascular research, Vol. 43, Issue 2, pp. 139-48, 2006
ImagesProduct Details anti-VEGF Antibody Target Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN181548) back to top