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|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling Images
E.coli derived recombinant Human VEGF165.
Type of Immunogen: Cell extract
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling Images back to top
|Alternative Name||VEGFA / VEGF (VEGFA Antibody Abstract)|
Name/Gene ID: VEGFA
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.05 - 0.1 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 5 μg/mL-15 μg/mL with a 30-minute incubation at room temperature or an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hVEGF (10 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody is required. ELISA: To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VEGF (LS-C104514) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top