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|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Epitope||AA 33-48, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|62 references available|
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Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN3044494) Images
|Purpose||Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vascular endothelial growth factor A
Protein Name: Vascular endothelial growth factor A(VEGF-A)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN3044494) Images back to top
|Alternative Name||VEGFA (VEGFA Antibody Abstract)|
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. Vascular endothelial growth factor(VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Vascular endothelial growth factor(VEGF) stimulates neurogenesis in vitro and in vivo.
Synonyms: MGC70609 antibody|Vascular endothelial growth factor A antibody|Vascular Endothelial Growth Factor antibody|Vascular Permeability Factor antibody|VEGF-A antibody|VEGFA antibody|VEGFA_HUMAN antibody|VPF antibody
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN3044494) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN3044494) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
References for anti-VEGF antibody (ABIN3044494)Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
|Product cited in:||
Zhang, Zhang, Yu, Du, Liu, Liu, An, Wang, Song, Li, Cao: "Effects of interferon tau on endometrial epithelial cells in caprine in vitro." in: Gene expression patterns : GEP, Vol. 25-26, pp. 142-148, 2017
Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." in: Carbohydrate polymers, Vol. 129, pp. 1-8, 2015
Jiang, Han, Li, Li, Yang, Liu: "Preparation and anti-tumor metastasis of carboxymethyl chitosan." in: Carbohydrate polymers, Vol. 125, pp. 53-60, 2015
Hou, Kong, Liang, Huang, Wen, Zheng, Wu, Chen: "HMGB1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts." in: Cellular & molecular immunology, Vol. 12, Issue 4, pp. 409-23, 2015
Liu, Su, Zhang, Wang, Qin, Liu, Tian, Qian: "Effect of Melilotus suaveolens extract on pulmonary microvascular permeability by downregulating vascular endothelial growth factor expression in rats with sepsis." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 3308-16, 2015
Wang, Lv, Zhang: "Combination of IL-24 and cisplatin inhibits angiogenesis and lymphangiogenesis of cervical cancer xenografts in a nude mouse model by inhibiting VEGF, VEGF-C and PDGF-B." in: Oncology reports, Vol. 33, Issue 5, pp. 2468-76, 2015
Song, Yue, Li, Li, Zhao, Zhang: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, 2014
Lu, Liu, Zhu, Xu: "Expression of nerve growth factor and hypoxia inducible factor-1? and its correlation with angiogenesis in non-small cell lung cancer." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 359-62, 2014
Duan, Lin, Li, Ding, Qian, Zhang, Ge, Fan, Li: "Effects of inhibition of hedgehog signaling on cell growth and migration of uveal melanoma cells." in: Cancer biology & therapy, Vol. 15, Issue 5, pp. 544-59, 2014
Zhan, Jia, Niu, Qi, Chen, Zhang, Zhang, Wang, Dong, Wang: "Targeted depletion of tumour-associated macrophages by an alendronate-glucomannan conjugate for cancer immunotherapy." in: Biomaterials, Vol. 35, Issue 38, pp. 10046-57, 2014
Liu, Wang, Qian, Li: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, 2014
Ge, Ding, Zhang, Li, Li: "Effect of angiogenesis inhibitor SU6668 in combination with 5-Fu on liver metastasis from transplantation tumors of human colorectal cancer in nude mice." in: International journal of clinical and experimental medicine, Vol. 7, Issue 10, pp. 3578-82, 2014
Ran, Ma, Liu, Tan, Liu, Lao: "Low protein diet inhibits uric acid synthesis and attenuates renal damage in streptozotocin-induced diabetic rats." in: Journal of diabetes research, Vol. 2014, pp. 287536, 2014
Liu, Yang, Zhang, Shui, Song, Yao, Dai, Sun: "Fructopyrano-(1?4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner." in: International journal of clinical and experimental medicine, Vol. 7, Issue 11, pp. 3859-69, 2014
Liu, Liu, Jiang, Xu, Cheng, Wang, Wang: "Preparation, antiangiogenic and antitumoral activities of the chemically sulfated glucan from Phellinus ribis." in: Carbohydrate polymers, Vol. 106, pp. 42-8, 2014
Ling, Deng, Mo, Zhang, Guan, Wei: "Correlations between CT perfusion parameters and vascular endothelial growth factor expression and microvessel density in implanted VX2 lung tumors." in: Cell biochemistry and biophysics, Vol. 70, Issue 1, pp. 629-33, 2014
Liu, Liu, Chen, Maitz, Chen, Huang: "Influence of a layer-by-layer-assembled multilayer of anti-CD34 antibody, vascular endothelial growth factor, and heparin on the endothelialization and anticoagulation of titanium surface." in: Journal of biomedical materials research. Part A, Vol. 101, Issue 4, pp. 1144-57, 2013
Zhou, Zhang, Li, Cui, Wang, Huang, Zheng, Lu, Chen: "Inhibition of vascular endothelial growth factor-mediated angiogenesis involved in reproductive toxicity induced by sesquiterpenoids of Curcuma zedoaria in rats." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 37, pp. 62-9, 2013
Zhang, Jia, Lin, Zhang, Dong, Zhao, Shen, Shen, Li, Yan, Li, Zhao, Xie: "AD-1, a novel ginsenoside derivative, shows anti-lung cancer activity via activation of p38 MAPK pathway and generation of reactive oxygen species." in: Biochimica et biophysica acta, Vol. 1830, Issue 8, pp. 4148-59, 2013
Chen, Wang, Li, Liu, Ni: "Comparative proteomics analysis of sodium selenite-induced apoptosis in human prostate cancer cells." in: Metallomics : integrated biometal science, Vol. 5, Issue 5, pp. 541-50, 2013
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