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|Antigen||Vascular Endothelial Growth Factor A (VEGFA) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This VEGF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-VEGF AntibodyTarget Details VEGF Application Details Handling References for anti-VEGF antibody (ABIN155190) Images
|Specificity||The antibody recognizes Human and Murine VEGF-A under reducing and non-reducing conditions.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human and Mouse.|
|Purification||Protein A Chromatography.|
|Immunogen||Recombinant Murine VEGF164 Dimer (Cat.-No DA3518)|
Target Details VEGFProduct Details anti-VEGF Antibody Application Details Handling References for anti-VEGF antibody (ABIN155190) Images back to top
|Alternative Name||VEGF-A (VEGFA Antibody Abstract)|
|Background||VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternate transcriptional splice variants, encoding either freely secreted or cell associated isoforms, have been characterized. There is also evidence for the use of non AUG (CUG) translation initiation sites upstream of, and in frame with the first AUG, leading to additional isoforms.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling|
|Pathways||RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-VEGF Antibody Target Details VEGF Handling References for anti-VEGF antibody (ABIN155190) Images back to top
ELISA: To detect Murine VEGF-A by direct ELISA a concentration of at least 0.5 μg/mL isrequired. This purified IgG, in combination with compatible secondary reagents, allows thedetection of 0.5- 1.0 ng/well of recombinant murine VEGF164 or other VEGF-A splice forms. Western blot: To detect murine VEGF-A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections (Pretreatment: 0.01 % Proteinase K, 15 min atRT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-VEGF Antibody Target Details VEGF Application Details References for anti-VEGF antibody (ABIN155190) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL|
|Buffer||PBS, pH 7.4 without preservatives and stabilizers|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-VEGF antibody (ABIN155190)Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling Images back to top
Kremer, Breier, Risau, Plate: "Up-regulation of flk-1/vascular endothelial growth factor receptor 2 by its ligand in a cerebral slice culture system." in: Cancer research, Vol. 57, Issue 17, pp. 3852-9, 1997
Fiebich, Jäger, Schöllmann, Weindel, Wilting, Kochs, Marmé, Hug, Weich: "Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells." in: European journal of biochemistry / FEBS, Vol. 211, Issue 1-2, pp. 19-26, 1993
Breier, Albrecht, Sterrer, Risau: "Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation." in: Development (Cambridge, England), Vol. 114, Issue 2, pp. 521-32, 1992