anti-Rabbit VEGF antibody for Immunofluorescence

Recommended VEGF Antibody (supplied by: Log in to see )

Antigen
Vascular Endothelial Growth Factor A (VEGFA) Antibodies
  • vegf
  • vegfa
  • wu:fj82c06
  • VEGF
  • vegf-a
  • vpf
  • vefg
  • VEGF-A
  • VPF
  • eVEGF120
  • eVEGF164
  • MVCD1
  • Vegf
  • Vegf120
  • Vegf164
  • Vegf188
  • Vpf
  • VEGF164
  • vascular endothelial growth factor Aa
  • vascular endothelial growth factor A L homeolog
  • vascular endothelial growth factor A
  • vegfaa
  • vegfa.L
  • VEGFA
  • vegfa
  • Vegfa
Reactivity
Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
282
116
112
24
19
16
14
8
3
3
3
2
1
1
1
1
1
1
Host
Mouse
261
89
24
4
2
1
1
Clonality
Monoclonal
Conjugate
This VEGF antibody is un-conjugated
42
23
12
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
288
170
83
61
26
26
19
17
17
15
12
11
7
5
4
2
2
1
1
1
Supplier
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Catalog No. ABIN3023875
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2629817 IF WB Goat IgG Log in to see Polyclonal 0
1 ABIN1502611 IF IP WB Mouse kappa, IgG1 Log in to see JH121 0
1 ABIN3023876 IF IHC (p) Mouse IgG1, kappa Log in to see VEGF-1063 0

General

Antigen Vascular Endothelial Growth Factor A (VEGFA) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(282), (116), (112), (24), (19), (16), (14), (8), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(261), (89), (24), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VEGF antibody is un-conjugated
(42), (23), (12), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(288), (170), (83), (61), (26), (26), (19), (17), (17), (15), (12), (11), (7), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-VEGF Antibody

Target Details VEGF Application Details Handling Images
Characteristics This mAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Purification Protein G affinity chromatography
Immunogen Human recombinant protein was used as the immunogen for the VEGF antibody.
Clone VEGF-1063
Isotype IgG1, kappa

Target Details VEGF

Product Details anti-VEGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name VEGFA (VEGFA Antibody Abstract)
Background This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-VEGF Antibody Target Details VEGF Handling Images back to top
Application Notes Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions For Research Use only

Handling

Product Details anti-VEGF Antibody Target Details VEGF Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-VEGF Antibody Target Details VEGF Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (ABIN3023875) Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with VEGF antibody ...
Immunohistochemistry (IHC) image for anti-Vascular Endothelial Growth Factor A (VEGFA) antibody (ABIN3023875) Formalin-fixed, paraffin-embedded human tonsil stained with VEGF antibody (VEGF/1063).