anti-Rat (Rattus) VEGFB antibody for Immunohistochemistry

Recommended VEGFB Antibody (supplied by: Log in to see )

Antigen

Vascular Endothelial Growth Factor B (VEGFB) Antibodies
show synonyms for this antigen

VEGFL
VRF
VEGF-B
Vrf
vascular endothelial growth factor B
VEGFB
Vegfb

Epitope

AA 22-207
Alternatives

Reactivity

Mouse (Murine), Rat (Rattus)
Alternatives

165

Host

Rabbit
Alternatives

Clonality

Conjugate

This VEGFB antibody is un-conjugated
Alternatives

Application

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives

175

100

Options

Supplier

Supplier Product No.

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Available images

Western Blotting (WB) image for anti-Vascular Endothelial Growth Factor B (VEGFB) (AA 22-207) antibody (ABIN5692963)

Immunohistochemistry (IHC) image for anti-Vascular Endothelial Growth Factor B (VEGFB) (AA 22-207) antibody (ABIN5692963)

Catalog No. ABIN5692963

$ 280.00
Plus shipping costs $45.00

Quantity

Price

100 μg

$280.00

Product detail page

Relevance Score	ABIN	Application	Host	Isotype	Epitope	Supplier	Clonality
8.933557	ABIN1875323	IHC WB	Rabbit	IgG		Log in to see	Polyclonal
8.933557	ABIN6265966	ELISA IHC WB	Rabbit	IgG		Log in to see	Polyclonal
8.933557	ABIN3023000	IHC WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN3187464	ELISA IHC WB	Rabbit	IgG	Internal Region	Log in to see	Polyclonal
1	ABIN1078651	ICC IHC WB	Rabbit	IgG	AA 21-137	Log in to see	Polyclonal
1	ABIN2973512	IHC WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN6265968	ELISA ICC IF IHC WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN2891733	IHC WB	Rabbit		Arg125	Log in to see	Polyclonal
1	ABIN2880126	IHC WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN2894559	IHC WB	Rabbit			Log in to see	Polyclonal
1	ABIN2384497	IC IHC ELISA WB	Rabbit	IgG	AA 21-137	Log in to see	Polyclonal
1	ABIN6091473	ELISA IHC WB	Rabbit	IgG	AA 22-207	Log in to see	Polyclonal
1	ABIN6104684	ELISA IHC WB	Rabbit	IgG	Internal Region	Log in to see	Polyclonal
1	ABIN2905457	IF/ICC IHC IP WB	Rabbit		AA 21-137	Log in to see	Polyclonal
1	ABIN5544849	IHC ELISA WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN2905575	ELISA IHC WB	Rabbit	IgG		Log in to see	Polyclonal
1	ABIN1994971	IHC WB	Rabbit	IgG		Log in to see
1	ABIN2858470	IHC WB	Rabbit			Log in to see	Polyclonal

More product categories related to VEGFB Antibodies

Similar anti-VEGFB Antibodies

Application / Reactivity	Human	Mouse (Murine)	Rat (Rattus)	Monkey	Cow (Bovine)	Dog (Canine)	Pig (Porcine)	Horse (Equine)	Guinea Pig
ELISA	65 antibody	20 antibody	20 antibody	2 antibody
Western Blotting (WB)	140 antibody	56 antibody	43 antibody	5 antibody	5 antibody	5 antibody	2 antibody	1 antibody	1 antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))	32 antibody	26 antibody	8 antibody	1 antibody
Immunoprecipitation (IP)	6 antibody	2 antibody	1 antibody		1 antibody	1 antibody
Proximity Ligation Assay (PLA)	1 antibody
Immunohistochemistry (IHC)	69 antibody	35 antibody	17 antibody	3 antibody	2 antibody	2 antibody
Immunofluorescence (Paraffin-embedded Sections) (IF (p))	14 antibody	14 antibody	14 antibody
Immunocytochemistry (ICC)	3 antibody	1 antibody	1 antibody		1 antibody	1 antibody
Immunohistochemistry (Frozen Sections) (IHC (fro))	1 antibody
Immunofluorescence (IF)	6 antibody
Immunochromatography (IC)	1 antibody		1 antibody
Enzyme Immunoassay (EIA)	1 antibody
Immunofluorescence (fixed cells) (IF/ICC)	6 antibody	2 antibody	1 antibody		1 antibody	1 antibody

General
Antigen	Vascular Endothelial Growth Factor B (VEGFB) Antibodies
Epitope	AA 22-207 Alternatives
	(16), (15), (10), (10), (7), (7), (6), (5), (5), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity	Mouse (Murine), Rat (Rattus) Alternatives
	(165), (73), (57), (5), (5), (5), (2), (1), (1)
Host	Rabbit Alternatives
	(121), (59), (19)
Clonality	Polyclonal
Conjugate	This VEGFB antibody is un-conjugated Alternatives
	(14), (13), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application	ELISA, Immunohistochemistry (IHC), Western Blotting (WB) Alternatives (175), (100), (68), (49), (14), (11), (11), (10), (9), (2), (1), (1), (1)
Supplier	Log in to see
Product Details anti-VEGFB Antibody Target Details VEGFB Application Details Handling Images
Brand	Picoband™
Cross-Reactivity (Details)	No cross reactivity with other proteins.
Characteristics	Rabbit IgG polyclonal antibody for VEGFB detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.
Immunogen	E. coli-derived rat VEGFB recombinant protein (Position: P22-A207).
Plasmids, Primers & others
Target Details VEGFB Product Details anti-VEGFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name	Vegfb (VEGFB Antibody Abstract)
Background	Synonyms: Vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF, Vegfb, Vrf Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.
UniProt	O35485
Pathways	RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Details Product Details anti-VEGFB Antibody Target Details VEGFB Handling Images back to top
Application Notes	Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). Application Details: Western blot, 0.1-0.5&mu,g/mL Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL Direct ELISA, 0.1-0.5&mu,g/mL
Restrictions	For Research Use only
Handling Product Details anti-VEGFB Antibody Target Details VEGFB Application Details Images back to top
Format	Lyophilized
Reconstitution	Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.
Preservative	Sodium azide
Precaution of Use	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage	4 °C,-20 °C
Storage Comment	At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Images Product Details anti-VEGFB Antibody Target Details VEGFB Application Details Handling back to top
Supplier Images	Western blot analysis of VEGFB using anti-VEGFB antibody . Electrophoresis was perfo... IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embe... IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embe... IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embe... IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embe... Images for product: anti-Vascular Endothelial Growth Factor B (VEGFB) (AA 22-207) antibody Western blot analysis of VEGFB using anti-VEGFB antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD. IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGFB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGFB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGFB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. IHC analysis of VEGFB using anti-VEGFB antibody . VEGFB was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-VEGFB Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Recommended VEGFB Antibody (supplied by: Log in to see )

Similar anti-VEGFB Antibodies

General

Product Details anti-VEGFB Antibody

Target Details VEGFB

Application Details

Handling

Images