anti-Mouse (Murine) VEGFR2 antibody for Western Blotting

Recommended VEGFR2 Antibody (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
  • CD309
  • FLK1
  • VEGFR
  • VEGFR2
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • sVEGFR-2
  • Vegfr-2
  • FLK-1
  • flk-1
  • vegfr-2
  • KDR
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • kinase insert domain receptor
  • kinase insert domain protein receptor
  • vascular endothelial growth factor receptor kdr-like
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • KDR
  • Kdr
  • LOC100136647
  • kdr
Epitope
C-Term, AA 1158-1365
64
60
57
39
25
23
22
19
16
16
15
15
15
13
13
12
12
11
10
10
10
9
7
6
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
599
367
179
25
7
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
519
146
60
4
2
1
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
43
32
25
24
16
12
11
9
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
458
250
218
202
125
102
42
29
26
25
19
17
11
9
9
8
7
6
5
2
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152058
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.232922 ABIN2506667 ICC IF IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.232922 ABIN783332 WB Rabbit AA 1057-1061 Log in to see Polyclonal 2
3.232922 ABIN2705327 IC IF IHC WB Rabbit C-Term, pTyr1175 Log in to see Polyclonal 0
3.232922 ABIN1533036 IHC ELISA WB Rabbit IgG AA 1141-1190 Log in to see Polyclonal 1
3.232922 ABIN1532428 ELISA WB Rabbit IgG AA 1025-1074 Log in to see Polyclonal 1
3.232922 ABIN1531411 ELISA WB Rabbit IgG AA 1025-1074, pTyr1059 Log in to see Polyclonal 1
3.232922 ABIN1531999 IHC ELISA WB Rabbit IgG AA 1180-1229, pTyr1214 Log in to see Polyclonal 1
3.232922 ABIN1533037 IHC ELISA WB Rabbit IgG AA 1180-1229 Log in to see Polyclonal 1
3.232922 ABIN1531998 IHC ELISA WB Rabbit IgG AA 1141-1190, pTyr1175 Log in to see Polyclonal 1
3.232922 ABIN1532000 IHC ELISA WB Rabbit IgG AA 917-966, pTyr951 Log in to see Polyclonal 0
3.232922 ABIN1533038 IHC ELISA WB Rabbit IgG AA 917-966 Log in to see Polyclonal 0
3.232922 ABIN652371 FACS WB Rabbit Ig Fraction AA 1153-1182, Tyr1175 Log in to see Polyclonal 2
3.232922 ABIN2180045 FACS IHC (p) WB Rabbit IgG AA 150-190 Log in to see Polyclonal 4
3.232922 ABIN151962 ICC IF WB Rabbit C-Term, AA 1300-1367 Log in to see Polyclonal 1
3.232922 ABIN408512 IHC (p) IHC WB Rabbit pTyr1214 Log in to see Polyclonal 0
3.232922 ABIN151961 ICC IF WB Rabbit Internal Region, AA 1200-1300 Log in to see Polyclonal 0
3.232922 ABIN1870315 IF IHC WB Rabbit IgG pTyr1214 Log in to see Polyclonal 0
3.232922 ABIN1870312 WB Rabbit IgG pTyr1059 Log in to see Polyclonal 0
3.232922 ABIN498249 WB Rabbit Log in to see Polyclonal 0
3.232922 ABIN3182009 ELISA IHC WB Rabbit IgG pTyr1175 Log in to see Polyclonal 0

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) Antibodies
Epitope C-Term, AA 1158-1365
(64), (60), (57), (39), (25), (23), (22), (19), (16), (16), (15), (15), (15), (13), (13), (12), (12), (11), (10), (10), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(599), (367), (179), (25), (7), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(519), (146), (60), (4), (2), (1), (1)
Clonality
Polyclonal
Conjugate This VEGFR2 antibody is un-conjugated
(43), (32), (25), (24), (16), (12), (11), (9), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(458), (250), (218), (202), (125), (102), (42), (29), (26), (25), (19), (17), (11), (9), (9), (8), (7), (6), (5), (2), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-VEGFR2 Antibody

Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) Images
Purification Unpurified
Immunogen Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]

Target Details VEGFR2

Product Details anti-VEGFR2 Antibody Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) Images back to top
Antigen
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Handling References for anti-VEGFR2 antibody (ABIN152058) Images back to top
Application Notes Western Blot 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 2-5 μL, Immunohistochemistry-Paraffin 1:10-1:500This VEGF Receptor 2 antibody is useful for Immunohistochemistry paraffin-embedded sections (PMID 10751359), Immunoprecipitation and Western blot where a doublet can been seen at ~ 150 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 20 µg of total transfected protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS with 0.5 % BSA for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details References for anti-VEGFR2 antibody (ABIN152058) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-VEGFR2 antibody (ABIN152058)

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling Images back to top
Product cited in:

Ying, Wang, Yang, Gu, Mai, Qiu, Chen, Wang: "Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway." in: Life sciences, Vol. 144, pp. 208-17, 2016 (Sample species: Human). Further details: Western Blotting

Tsuboi, Yamashita, Nagano, Kimura, Toa Salazar, Ohneda: "Impaired expression of HIF-2α induces compensatory expression of HIF-1α for the recovery from anemia." in: Journal of cellular physiology, Vol. 230, Issue 7, pp. 1534-48, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

André, Tunik, Aronsson, Kvanta: "Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model." in: Investigative ophthalmology & visual science, Vol. 56, Issue 11, pp. 6591-604, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008

Singh, Meyer, Band, Rahimi: "The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 2106-18, 2005 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Meyer, Singh, Rahimi: "The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling." in: The Journal of biological chemistry, Vol. 279, Issue 1, pp. 735-42, 2003 Further details: Western Blotting,Immunoprecipitation

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Lashkari, Hirose, Yazdany, McMeel, Kazlauskas, Rahimi: "Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity." in: The American journal of pathology, Vol. 156, Issue 4, pp. 1337-44, 2000 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-VEGFR2 Antibody Target Details VEGFR2 Application Details Handling References for anti-VEGFR2 antibody (ABIN152058) back to top
Supplier Images
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody (ABIN152058) anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody (ABIN152058) Western Blot: VEGF R2/KDR/Flk-1 Antibody [ABIN152058] - Analysis of VEGF R2/KDR/Flk-1...
Western Blotting (WB) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody (ABIN152058) Western Blot: VEGF R2/KDR/Flk-1 Antibody - Lane 1 is 20 ug , lane 2 is 40 ug and lan...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody (ABIN152058) Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody - IHC analysis of formalin...