Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed

Catalog No. ABIN964964

Supplier Product No.: 610-156-121s

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Atto 647N

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-156-121s

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.

Characteristics: Anti-Mouse IgG3 ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).

Purification: Mouse IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse IgG ATTO 647N Antibody has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Fernandes, Loubens, Marinach, Coppée, Baron, Grand, Andre, Hamada, Langlois, Briquet, Bun, Silvie: "Plasmodium sporozoites require the protein B9 to invade hepatocytes." in: iScience, Vol. 26, Issue 2, pp. 106056, (2023) (PubMed).

Maidorn, Olichon, Rizzoli, Opazo: "Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons." in: mAbs, Vol. 11, Issue 2, pp. 305-321, (2019) (PubMed).

Richter, Wildhagen, Helm, Ußling, Schikorski, Rizzoli: "Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins." in: Scientific reports, Vol. 8, Issue 1, pp. 14838, (2018) (PubMed).

Purkey, Woolfrey, Crosby, Stich, Chick, Aoto, DellAcqua: "AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca2+-Permeable AMPA Receptors to Control LTP." in: Cell reports, Vol. 25, Issue 4, pp. 974-987.e4, (2018) (PubMed).

Gomes de Castro, Höbartner, Opazo: "Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy." in: PLoS ONE, Vol. 12, Issue 2, pp. e0173050, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.