anti-Zebrafish (Danio rerio) E-cadherin antibody for Western Blotting

Recommended E-cadherin Antibody (supplied by: Log in to see )

Antigen
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antibodies
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • cdh1
  • Cdh1
  • cdh1.S
Epitope
Middle Region
98
35
26
21
19
16
16
15
15
14
10
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
797
238
143
40
25
15
8
7
7
6
6
4
3
1
1
1
1
1
1
1
1
Host
Rabbit
543
264
37
22
2
Clonality
Polyclonal
Conjugate
This E-cadherin antibody is un-conjugated
43
39
34
31
24
21
21
17
17
15
13
13
13
13
12
12
11
10
8
7
7
6
4
3
3
3
3
3
3
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
645
371
275
213
192
176
157
87
81
38
19
14
13
12
10
7
6
6
4
2
2
2
2
2
1
1
1
Options
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Catalog No. ABIN2784498
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2908103 ABIN2787999 WB Rabbit Middle Region Log in to see Polyclonal 0
3.2908103 ABIN2787998 WB Rabbit C-Term Log in to see Polyclonal 0
3.2908103 ABIN2704739 IC FACS IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2618875 FACS ICC IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5618640 FACS IHC IHC (fro) WB Mouse IgG2b Log in to see MB2 0
1 ABIN2994235 FACS IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antibodies
Epitope Middle Region
(98), (35), (26), (21), (19), (16), (16), (15), (15), (14), (10), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(797), (238), (143), (40), (25), (15), (8), (7), (7), (6), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(543), (264), (37), (22), (2)
Clonality
Polyclonal
Conjugate This E-cadherin antibody is un-conjugated
(43), (39), (34), (31), (24), (21), (21), (17), (17), (15), (13), (13), (13), (13), (12), (12), (11), (10), (8), (7), (7), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(645), (371), (275), (213), (192), (176), (157), (87), (81), (38), (19), (14), (13), (12), (10), (7), (6), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-E-cadherin Antibody

Target Details E-cadherin Application Details Handling References for anti-E-cadherin antibody (ABIN2784498) Images
Blocking Peptide Blocking peptide for this product available: ABIN974746
Sequence QEITSYTAQE PDTFMEQKIT YRIWRDTANW LEINPDTGAI STRAELDRED
Predicted Reactivity Cow: 93%, Dog: 79%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 93%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against CDH1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CDH1

Target Details E-cadherin

Product Details anti-E-cadherin Antibody Application Details Handling References for anti-E-cadherin antibody (ABIN2784498) Images back to top
Antigen
Alternative Name CDH1 (CDH1 Antibody Abstract)
Background Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: Arc-1, CD324, CDHE, ECAD, LCAM, UVO
Protein Interaction Partner: UBC, MDM2, UCHL3, CDC27, PTEN, CTNNB1, PIP5K1C, CTNND1, CBLL1, RNF2, GMNN, LMNA, KRT18, KRT9, KRT8, KRT1, IGHA1, HSPD1, HSP90AA1, HSPA9, HSPA8, HSPA5, GBAS, CTNNA1, CRYAB, CFTR, C1QBP, ACTN4, ACADVL, ANP32B, NIPSNAP1, XRCC5, TGM2, SKP2, SET, RAD17, POLR2E
Protein Size: 882
Molecular Weight 80 kDa
Gene ID 999
NCBI Accession NM_004360, NP_004351
UniProt P12830
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

Application Details

Product Details anti-E-cadherin Antibody Target Details E-cadherin Handling References for anti-E-cadherin antibody (ABIN2784498) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 882 AA

Restrictions For Research Use only

Handling

Product Details anti-E-cadherin Antibody Target Details E-cadherin Application Details References for anti-E-cadherin antibody (ABIN2784498) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-E-cadherin antibody (ABIN2784498)

Product Details anti-E-cadherin Antibody Target Details E-cadherin Application Details Handling Images back to top
Background publications

Onder, Gupta, Mani, Yang, Lander, Weinberg: "Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways." in: Cancer research, Vol. 68, Issue 10, pp. 3645-54, 2008

Images

Product Details anti-E-cadherin Antibody Target Details E-cadherin Application Details Handling References for anti-E-cadherin antibody (ABIN2784498) back to top
Supplier Images
Western Blotting (WB) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (ABIN2784498) WB Suggested Anti-CDH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (ABIN2784498) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (ABIN2784498) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (Image 3)
Immunofluorescence (IF) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Middle Region) antibody (ABIN2784498) Sample Type: Rat FRTL-5 cells

Primary Antibody Dilution: 1:100