anti-Human FLT1 antibody for Western Blotting

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Epitope
AA 1160-1210
43
34
17
15
14
12
12
8
8
7
6
6
5
5
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
435
148
113
17
17
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
253
249
13
5
3
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
35
20
10
10
10
9
9
7
7
7
6
6
6
6
6
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
306
196
149
102
102
76
60
40
22
13
12
10
9
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN725795
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0806355 ABIN151960 ICC IF IHC IHC (p) WB Rabbit Internal Region, AA 800-900 Log in to see Polyclonal 5
3.0806355 ABIN2705326 WB Rabbit C-Term, pTyr1213 Log in to see Polyclonal 0
3.0806355 ABIN1531410 IHC ELISA WB Rabbit IgG AA 1289-1338, pTyr1333 Log in to see Polyclonal 1
3.0806355 ABIN6265971 ELISA ICC IF IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
3.0806355 ABIN269286 ICC IF IP ELISA WB Mouse IgG1 Log in to see EWF 0
3.0806355 ABIN2869445 ELISA WB Mouse IgG1 Log in to see 3D10 1
3.0806355 ABIN2788655 WB Rabbit C-Term Log in to see Polyclonal 0
3.0806355 ABIN3183067 ELISA WB Rabbit IgG pTyr1213 Log in to see Polyclonal 0
3.0806355 ABIN3182563 ELISA IHC WB Rabbit IgG pTyr1333 Log in to see Polyclonal 0
3.0806355 ABIN955511 EIA FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 1
3.0806355 ABIN3042716 WB Rabbit IgG AA 1299-1317, C-Term Log in to see Polyclonal 0
3.0806355 ABIN269284 ELISA ICC IF WB Mouse IgG1 Log in to see Flt-1-EWC 1
3.0806355 ABIN969146 ELISA WB Mouse IgG1 Log in to see 3D10 2
3.0806355 ABIN5961612 ELISA IHC (p) WB Rabbit IgG Tyr1048 Log in to see Polyclonal 0
3.0806355 ABIN5961613 ELISA IHC (p) WB Rabbit IgG Tyr1333 Log in to see Polyclonal 0
3.0806355 ABIN5961614 ELISA IHC (p) WB Rabbit IgG pTyr1048 Log in to see Polyclonal 0
3.0806355 ABIN5961615 ELISA WB Rabbit IgG pTyr1213 Log in to see Polyclonal 0
3.0806355 ABIN5961616 ELISA IHC (p) WB Rabbit IgG pTyr1333 Log in to see Polyclonal 0
3.0806355 ABIN1175060 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-328 Log in to see Polyclonal 0
3.0806355 ABIN5693264 WB Rabbit Log in to see Polyclonal 0

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope AA 1160-1210
(43), (34), (17), (15), (14), (12), (12), (8), (8), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(435), (148), (113), (17), (17), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(253), (249), (13), (5), (3), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(35), (20), (10), (10), (10), (9), (9), (7), (7), (7), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(306), (196), (149), (102), (102), (76), (60), (40), (22), (13), (12), (10), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN725795) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat VEGFR1
Isotype IgG
Plasmids, Primers & others

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 antibody (ABIN725795) Images back to top
Antigen
Alternative Name VEGFR1 (FLT1 Antibody Abstract)
Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity, may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).

Subcellular location: Cytoplasm

Synonyms: VEGFR-1, Vascular endothelial growth factor receptor 1, Fms-like tyrosine kinase 1, FLT-1, Tyrosine-protein kinase receptor FLT, Flt1, Vegfr1

Gene ID 54251
UniProt P53767
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 antibody (ABIN725795) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 antibody (ABIN725795) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-FLT1 antibody (ABIN725795)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Liu, Zou, Li, Wang, Wang, Hao, Ke, Li: "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." in: International journal of molecular medicine, Vol. 40, Issue 1, pp. 65-74, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Cheng, Xiang, Yang, Ma, Zhao: "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." in: Cell biochemistry and biophysics, 2015 (Sample species: Rat (Rattus)).

Liu, Wang, Wang, Wang, Xue: "Paeoniflorin attenuates A?1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-?B- and VEGF/Flt-1 signaling pathways." in: Brain research, Vol. 1618, pp. 149-58, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Brain

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN725795) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1/FLT1 P...
SDS-PAGE (SDS) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Rat lung lysates 30ug, probed (ABIN725795) at 1:200 overnight in 4 °C. Followed by co...
Immunofluorescence (IF) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Image kindly submitted by Victor Blanco as part of the Free Sample Program. Frozen mo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1/FLT1 P...
Western Blotting (WB) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Rat lung lysates 30ug, probed at 1:200 overnight in 4˚C. Followed by conjugation to ...
 image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 1160-1210) antibody (ABIN725795) Mouse spleen cells probed with VEGFR1 Polyclonal Antibody, unconjugated at 1:100 dil...