anti-Rat (Rattus) GRIN1 antibody for Immunoprecipitation

Recommended GRIN1 Antibody (supplied by: Log in to see )

Antigen
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) Antibodies
  • GluN1
  • MRD8
  • NMDA1
  • NMDAR1
  • NR1
  • nmda1
  • nmdar1
  • GB19253
  • GRIN1
  • NMD-R1
  • GluRdelta1
  • GluRzeta1
  • M100174
  • Nmdar
  • Rgsc174
  • NMDAR1.1
  • zgc:152678
  • glutamate ionotropic receptor NMDA type subunit 1
  • glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog
  • NMDA receptor 1
  • glutamate receptor, ionotropic, NMDA1 (zeta 1)
  • glutamate receptor, ionotropic, N-methyl D-aspartate 1a
  • GRIN1
  • grin1.S
  • grin1
  • Grin1
  • Nmdar1
  • grin1a
Epitope
AA 1-564
43
34
32
22
18
15
15
11
11
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
244
244
191
14
5
5
4
3
3
2
2
2
1
1
Host
Mouse
242
51
13
Clonality
Monoclonal
Conjugate
This GRIN1 antibody is un-conjugated
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
231
149
89
40
36
24
22
13
10
9
2
2
2
1
Options
Supplier
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Catalog No. ABIN361405
$ 508.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.340845 ABIN152467 ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see R1JHL 11
13.297794 ABIN1690149 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
13.297794 ABIN1690497 IP Sepharose Rabbit Log in to see Polyclonal 0
4 ABIN210530 IP IHC WB Mouse AA 1-564 Log in to see R1JHL 0
4 ABIN5579352 IP WB Mouse Log in to see R1JHL 0
1 ABIN210934 ICC IP WB Mouse IgG AA 834-938 Log in to see 0
1 ABIN602062 IP IHC WB Rabbit IgG pSer897 Log in to see Polyclonal 0
1 ABIN1495175 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1495176 IP Sepharose Rabbit N-Term Log in to see Polyclonal 0
1 ABIN210532 IP WB Mouse IgG AA 1-564 Log in to see 0
1 ABIN2312556 IC IP WB Mouse IgG C-Term, AA 834-938 Log in to see 0-T-190 0
1 ABIN2312559 IP IHC WB Rabbit IgG pSer897 Log in to see Polyclonal 0
1 ABIN6333515 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN6341515 IF/ICC IHC IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) Antibodies
Epitope AA 1-564
(43), (34), (32), (22), (18), (15), (15), (11), (11), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(244), (244), (191), (14), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(242), (51), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GRIN1 antibody is un-conjugated
(7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(231), (149), (89), (40), (36), (24), (22), (13), (10), (9), (2), (2), (2), (1)
Pubmed 11 references available
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Product Details anti-GRIN1 Antibody

Target Details GRIN1 Application Details Handling References for anti-GRIN1 antibody (ABIN361405) Images
Specificity Specific for the ~120k NR1 subunit of the NMDA receptor. The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Culture supernatant
Immunogen Fusion protein containing amino acids 1-564 of the NR1 subunit
Clone R1JHL
Plasmids, Primers & others

Target Details GRIN1

Product Details anti-GRIN1 Antibody Application Details Handling References for anti-GRIN1 antibody (ABIN361405) Images back to top
Antigen
Alternative Name GRIN1 (GRIN1 Antibody Abstract)
Background The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDAR, NR1 Subunit Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor.
Molecular Weight '120 kDa
Gene ID 24408
UniProt P35439
Pathways Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-GRIN1 Antibody Target Details GRIN1 Handling References for anti-GRIN1 antibody (ABIN361405) Images back to top
Application Notes Recommended Dilution: WB: 1:1000 IP: 3 µg per 200 µg lysate
Restrictions For Research Use only

Handling

Product Details anti-GRIN1 Antibody Target Details GRIN1 Application Details References for anti-GRIN1 antibody (ABIN361405) Images back to top
Format Lyophilized
Buffer Lyophilized
Storage -20 °C

References for anti-GRIN1 antibody (ABIN361405)

Product Details anti-GRIN1 Antibody Target Details GRIN1 Application Details Handling Images back to top
Product cited in:

Quillinan, Grewal, Deng, Shimizu, Yonchek, Strnad, Traystman, Herson: "Region-specific role for GluN2B-containing NMDA receptors in injury to Purkinje cells and CA1 neurons following global cerebral ischemia." in: Neuroscience, Vol. 284, pp. 555-65, 2014

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, 2011

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, 2008

Zhang, Dong, Zhang, Ichinose, Wu, Culley, Crosby, Tanzi, Xie: "Isoflurane-induced caspase-3 activation is dependent on cytosolic calcium and can be attenuated by memantine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 17, pp. 4551-60, 2008

Background publications

Alvestad, Grosshans, Coultrap, Nakazawa, Yamamoto, Browning: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, 2003

Wenthold, Prybylowski, Standley, Sans, Petralia: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003

Carroll, Zukin: "NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity." in: Trends in neurosciences, Vol. 25, Issue 11, pp. 571-7, 2002

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, 2001

Lu, Xiong, Lei, Orser, Dudek, Browning, MacDonald: "G-protein-coupled receptors act via protein kinase C and Src to regulate NMDA receptors." in: Nature neuroscience, Vol. 2, Issue 4, pp. 331-8, 1999

Snell, Nunley, Lickteig, Browning, Tabakoff, Hoffman: "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion." in: Brain research. Molecular brain research, Vol. 40, Issue 1, pp. 71-8, 1997

Lovinger, White, Weight: "Ethanol inhibits NMDA-activated ion current in hippocampal neurons." in: Science (New York, N.Y.), Vol. 243, Issue 4899, pp. 1721-4, 1989

Images

Product Details anti-GRIN1 Antibody Target Details GRIN1 Application Details Handling References for anti-GRIN1 antibody (ABIN361405) back to top
Supplier Images
Western Blotting (WB) image for anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 1-564) antibody (ABIN361405) Western blots of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeli...
Western Blotting (WB) image for anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) (AA 1-564) antibody (ABIN361405) Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of th...