anti-Human Neuroligin 1 antibody for Immunofluorescence

Recommended Neuroligin 1 Antibody (supplied by: Log in to see )

Antigen
Neuroligin 1 (NLGN1) Antibodies
  • NLGN1
  • Nlgn1
  • GB18720
  • nlgn1
  • NL1
  • 6330415N05Rik
  • BB179718
  • mKIAA1070
  • neuroligin-1
  • neuroligin 1
  • NLGN1
  • LOC659779
  • LOC724358
  • nlgn1
  • Nlgn1
Epitope
Internal Region
72
21
15
10
4
4
3
2
2
1
1
1
1
1
1
Reactivity
Human
105
88
80
3
2
2
1
1
1
1
1
1
1
Host
Goat
87
35
13
Clonality
Polyclonal
Conjugate
This Neuroligin 1 antibody is un-conjugated
8
8
7
6
5
5
5
5
4
4
4
4
4
4
4
4
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF)
101
79
48
41
24
19
13
11
2
Options
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Available images

Catalog No. ABIN185446
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.297794 ABIN1741306 ICC IF IHC WB Atto 390 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741313 ICC IF IHC WB Atto 700 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741310 ICC IF IHC WB Atto 633 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741311 ICC IF IHC WB Atto 655 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741312 ICC IF IHC WB Atto 680 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741315 ICC IF IHC WB APC Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741320 ICC IF IHC WB PerCP Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741319 ICC IF IHC WB PE-Atto 594 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741317 ICC IF IHC WB FITC Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741307 ICC IF IHC WB Atto 488 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741308 ICC IF IHC WB Atto 565 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741309 ICC IF IHC WB Atto 594 Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741316 ICC IF IHC WB Biotin Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741314 ICC IF IHC WB Alkaline Phosphatase (AP) Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741318 ICC IF IHC WB HRP Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741321 ICC IF IHC WB PE Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741322 ICC IF IHC WB Streptavidin Mouse IgG1 AA 718-843 Log in to see S97A-31 0
19.297794 ABIN1741305 ICC IF IHC WB Mouse IgG1 AA 718-843 Log in to see S97A-31 0
1 ABIN5540525 ICC IF IHC (p) WB Mouse IgG1 AA 718-843, C-Term, Cytoplasmic Domain Log in to see S97A-31 0

General

Antigen Neuroligin 1 (NLGN1) Antibodies
Epitope Internal Region
(72), (21), (15), (10), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(105), (88), (80), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(87), (35), (13)
Clonality
Polyclonal
Conjugate This Neuroligin 1 antibody is un-conjugated
(8), (8), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF)
(101), (79), (48), (41), (24), (19), (13), (11), (2)
Pubmed 1 reference available
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Product Details anti-Neuroligin 1 Antibody

Target Details Neuroligin 1 Application Details Handling References for anti-Neuroligin 1 antibody (ABIN185446) Images
Purpose Neuroligin 1
Blocking Peptide Blocking peptide for this product available: ABIN369952
Sequence TAKQDDPKQQ
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-TAKQDDPKQQ, from the internal region of the protein sequence according to NP_055747.1.
Plasmids, Primers & others

Target Details Neuroligin 1

Product Details anti-Neuroligin 1 Antibody Application Details Handling References for anti-Neuroligin 1 antibody (ABIN185446) Images back to top
Antigen
Alternative Name NLGN1 (NLGN1 Antibody Abstract)
Background NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
Gene ID 22871
NCBI Accession NP_055747
Pathways Synaptic Membrane, Synaptic Vesicle Exocytosis

Application Details

Product Details anti-Neuroligin 1 Antibody Target Details Neuroligin 1 Handling References for anti-Neuroligin 1 antibody (ABIN185446) Images back to top
Application Notes

DS WB Results: Preliminary experiments gave an approx 150 kDa band in Human Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:4000.

Comment

Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions For Research Use only

Handling

Product Details anti-Neuroligin 1 Antibody Target Details Neuroligin 1 Application Details References for anti-Neuroligin 1 antibody (ABIN185446) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-Neuroligin 1 antibody (ABIN185446)

Product Details anti-Neuroligin 1 Antibody Target Details Neuroligin 1 Application Details Handling Images back to top
Background publications

Chih, Engelman, Scheiffele: "Control of excitatory and inhibitory synapse formation by neuroligins." in: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, 2005

Images

Product Details anti-Neuroligin 1 Antibody Target Details Neuroligin 1 Application Details Handling References for anti-Neuroligin 1 antibody (ABIN185446) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Neuroligin 1 (NLGN1) (Internal Region) antibody (ABIN185446) ABIN185446 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabi...
Immunofluorescence (IF) image for anti-Neuroligin 1 (NLGN1) (Internal Region) antibody (ABIN185446) ABIN185446 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabi...