anti-Mouse (Murine) NMDAR2A antibody for Immunohistochemistry (Frozen Sections)

Recommended NMDAR2A Antibody (supplied by: Log in to see )

Antigen
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) Antibodies
  • NMDAR2A
  • nr2a
  • GRIN2A
  • EPND
  • GluN2A
  • NR2A
  • glutamate ionotropic receptor NMDA type subunit 2A
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog
  • glutamate receptor ionotropic, NMDA 2A
  • glutamate receptor, ionotropic, NMDA2A (epsilon 1)
  • GRIN2A
  • grin2a.L
  • LOC100438553
  • Grin2a
Epitope
C-Term
87
29
27
19
15
11
10
10
8
8
7
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
211
173
172
7
4
3
2
2
1
1
1
Host
Rabbit
173
106
1
Clonality
Polyclonal
Conjugate
This NMDAR2A antibody is un-conjugated
16
16
14
11
9
9
7
5
5
5
5
5
5
5
5
5
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
236
177
63
60
53
34
26
26
21
15
3
2
2
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN5551596
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551598 IF IHC (fro) WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) Antibodies
Epitope C-Term
(87), (29), (27), (19), (15), (11), (10), (10), (8), (8), (7), (5), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(211), (173), (172), (7), (4), (3), (2), (2), (1), (1), (1)
Host Rabbit
(173), (106), (1)
Clonality
Polyclonal
Conjugate This NMDAR2A antibody is un-conjugated
(16), (16), (14), (11), (9), (9), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(236), (177), (63), (60), (53), (34), (26), (26), (21), (15), (3), (2), (2), (1)
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Product Details anti-NMDAR2A Antibody

Target Details NMDAR2A Application Details Handling Images
Specificity This antibody recognizes the ~180k NR2A subunit of the NMDA Receptor in Western Blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography.
Immunogen Fusion protein from the C-terminus of the NR2A subunit of Rat NMDA Receptor.
Isotype IgG
Plasmids, Primers & others

Target Details NMDAR2A

Product Details anti-NMDAR2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name NMDA Receptor 2A (GRIN2A Antibody Abstract)
Background The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A
Gene ID 2903
NCBI Accession NP_000824
UniProt Q12879
Pathways Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-NMDAR2A Antibody Target Details NMDAR2A Handling Images back to top
Application Notes Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000. Immunoprecipitation: 3 μL per 200 μg lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NMDAR2A Antibody Target Details NMDAR2A Application Details Images back to top
Reconstitution Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Buffer 5 mM Ammonium Bicarbonate.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.