anti-Mouse (Murine) NMDAR2A antibody for Immunoprecipitation

Recommended NMDAR2A Antibody

Antigen
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) Antibodies
  • NMDAR2A
  • nr2a
  • GRIN2A
  • EPND
  • GluN2A
  • NR2A
  • glutamate ionotropic receptor NMDA type subunit 2A
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog
  • glutamate receptor ionotropic, NMDA 2A
  • glutamate receptor, ionotropic, NMDA2A (epsilon 1)
  • GRIN2A
  • grin2a.L
  • LOC100438553
  • Grin2a
Epitope
C-Term
118
57
26
19
17
15
10
9
8
8
8
8
7
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
270
218
217
7
4
3
2
2
1
1
1
Host
Rabbit
199
137
1
Clonality
Polyclonal
Conjugate
This NMDAR2A antibody is un-conjugated
21
17
15
12
10
10
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
265
206
76
67
59
43
33
26
20
17
3
2
2
2
Options
Catalog No. ABIN5551596
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN211363 IP WB Rabbit IgG AA 1265-1464 Polyclonal 0
1 ABIN2312809 IP WB Rabbit IgG AA 1265-1464 Polyclonal 0
1 ABIN5541473 IP WB Rabbit Ig Fraction C-Term Polyclonal 0
1 ABIN6341519 IF/ICC IHC IP WB Rabbit Polyclonal 0
1 ABIN6031368 IF/ICC IHC IP WB Rabbit Polyclonal 0
-13.054157 ABIN4340158 IHC IHC (p) IP WB Rabbit C-Term Polyclonal 0

General

Antigen Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) Antibodies
Epitope C-Term
(118), (57), (26), (19), (17), (15), (10), (9), (8), (8), (8), (8), (7), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(270), (218), (217), (7), (4), (3), (2), (2), (1), (1), (1)
Host Rabbit
(199), (137), (1)
Clonality
Polyclonal
Conjugate This NMDAR2A antibody is un-conjugated
(21), (17), (15), (12), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(265), (206), (76), (67), (59), (43), (33), (26), (20), (17), (3), (2), (2), (2)
Pubmed 6 references available
Supplier

Product Details anti-NMDAR2A Antibody

Target Details NMDAR2A Application Details Handling References for anti-NMDAR2A antibody (ABIN5551596) Images
Specificity This antibody recognizes the ~180k NR2A subunit of the NMDA Receptor in Western Blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography.
Immunogen Fusion protein from the C-terminus of the NR2A subunit of Rat NMDA Receptor.
Isotype IgG
Plasmids, Primers & others

Target Details NMDAR2A

Product Details anti-NMDAR2A Antibody Application Details Handling References for anti-NMDAR2A antibody (ABIN5551596) Images back to top
Antigen
Alternative Name NMDA Receptor 2A (GRIN2A Antibody Abstract)
Background The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A
Gene ID 2903
NCBI Accession NP_000824
UniProt Q12879
Pathways Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-NMDAR2A Antibody Target Details NMDAR2A Handling References for anti-NMDAR2A antibody (ABIN5551596) Images back to top
Application Notes Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000. Immunoprecipitation: 3 μL per 200 μg lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NMDAR2A Antibody Target Details NMDAR2A Application Details References for anti-NMDAR2A antibody (ABIN5551596) Images back to top
Reconstitution Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Buffer 5 mM Ammonium Bicarbonate.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-NMDAR2A antibody (ABIN5551596)

Product Details anti-NMDAR2A Antibody Target Details NMDAR2A Application Details Handling Images back to top
Product cited in:

Bostrom, Majaess, Morch, White, Eadie, Christie: "Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 25, Issue 1, pp. 271-9, 2014

Pozniak, Sengupta Ghosh, Gogineni, Hanson, Lee, Larson, Solanoy, Bustos, Li, Ngu, Jubb, Ayalon, Wu, Scearce-Levie, Zhou, Weimer, Kirkpatrick, Lewcock: "Dual leucine zipper kinase is required for excitotoxicity-induced neuronal degeneration." in: The Journal of experimental medicine, Vol. 210, Issue 12, pp. 2553-67, 2013

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, 2011

Norris, Strickland: "Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 33, pp. 13473-8, 2007

Alvestad, Grosshans, Coultrap, Nakazawa, Yamamoto, Browning: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, 2003

Wenthold, Prybylowski, Standley, Sans, Petralia: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003