anti-Human Aurora Kinase B antibody for Western Blotting

Recommended Aurora Kinase B Antibody

Antigen
Aurora Kinase B (AURKB) Antibodies
  • AIK2
  • AIM-1
  • AIM1
  • ARK2
  • AurB
  • IPL1
  • PPP1R48
  • STK12
  • STK5
  • aurkb-sv1
  • aurkb-sv2
  • AIRK2
  • AL022959
  • Aik2
  • Aim1
  • Ark2
  • STK-1
  • Stk12
  • Stk5
  • Aur B
  • Aurora B
  • Aurora-B
  • CG6620
  • DmAIRK2
  • DmAurB
  • DmAuroraB
  • Dmel\\CG6620
  • IAL
  • IAL1
  • Ial
  • aurB
  • aurora B
  • ARK-2
  • XAIRK2
  • aik2
  • aim-1
  • aim1
  • airk2
  • ark2
  • ipl1
  • stk12
  • stk5
  • CHUNP6896
  • fa09g06
  • stka
  • wu:fa09g06
  • aurkb
  • AIRK2-B
  • XAIRK2-B
  • aurkb-a
  • aurkb-b
  • xAurora-B-B
  • aurora kinase B
  • aurora B
  • aurora kinase B S homeolog
  • AURKB
  • Aurkb
  • aurB
  • aurkb
  • aurkb.S
Epitope
AA 223-234, pThr232
44
32
27
21
16
13
12
10
10
9
7
7
7
7
6
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
291
120
92
17
8
7
4
2
2
2
1
1
1
Host
Rabbit
217
70
7
7
Clonality
Polyclonal
Conjugate
This Aurora Kinase B antibody is un-conjugated
12
9
9
8
8
7
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
231
142
107
93
43
32
15
12
11
10
10
8
2
2
1
Options

Available images

Catalog No. ABIN129612
$ 598.50
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
21.839296 ABIN1724660 ELISA WB Mouse IgG1 13E8D3 2
20.947433 ABIN129590 ELISA WB Rabbit IgG AA 227-238 Polyclonal 1
19.891861 ABIN3030076 IHC ELISA WB Rabbit Ig Fraction AA 6-35 Polyclonal 0
17.947433 ABIN965626 ELISA WB IgG1 2
17.339296 ABIN3183366 ELISA IF IHC WB Rabbit IgG Tyr436 Polyclonal 0
17.339296 ABIN3183367 ELISA IF IHC WB Rabbit IgG Tyr434, Tyr437 Polyclonal 0
17.339296 ABIN931443 ELISA WB Mouse IgG1 13E8D3 0
16.891861 ABIN563978 IF IP ELISA WB Mouse IgG1 kappa AA 1-90 6H7 0
13.891861 ABIN6256516 ELISA ICC IF IHC WB Rabbit IgG pThr232 Polyclonal 0
13.891861 ABIN6260155 ELISA ICC IF WB Rabbit IgG Polyclonal 0
13.891861 ABIN563979 RNAi ELISA WB Mouse IgG1 kappa AA 1-90 6G8 0
13.447434 ABIN2613393 ELISA WB Goat Internal Region Polyclonal 0
13.447434 ABIN3182642 ELISA IHC WB Rabbit IgG pTyr12 Polyclonal 0
13.447434 ABIN3182806 ELISA IF IHC WB Rabbit IgG pThr232 Polyclonal 0
13.447434 ABIN4901009 ELISA WB Mouse IgG1 13E8D3 0
13.447434 ABIN6933900 WB Mouse IgG1, kappa AA 89-251 AURKB-1521 0
13.447434 ABIN5617430 ELISA IF IHC WB Rabbit IgG pThr232 Polyclonal 0
13.447434 ABIN5617432 ELISA IF IHC WB Rabbit IgG Tyr12 Polyclonal 0
13.447434 ABIN6936440 WB Mouse IgG1, kappa AA 89-251 AURKB-1521 0
13.447434 ABIN5617431 ELISA IF WB Rabbit IgG Thr232 Polyclonal 0

General

Antigen Aurora Kinase B (AURKB) Antibodies
Epitope AA 223-234, pThr232
(44), (32), (27), (21), (16), (13), (12), (10), (10), (9), (7), (7), (7), (7), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(291), (120), (92), (17), (8), (7), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(217), (70), (7), (7)
Clonality
Polyclonal
Conjugate This Aurora Kinase B antibody is un-conjugated
(12), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(231), (142), (107), (93), (43), (32), (15), (12), (11), (10), (10), (8), (2), (2), (1)
Pubmed 12 references available
Supplier

Product Details anti-Aurora Kinase B Antibody

Target Details Aurora Kinase B Application Details Handling References for anti-Aurora Kinase B antibody (ABIN129612) Images
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.
Isotype IgG
Plasmids, Primers & others

Target Details Aurora Kinase B

Product Details anti-Aurora Kinase B Antibody Application Details Handling References for anti-Aurora Kinase B antibody (ABIN129612) Images back to top
Antigen
Alternative Name Aurora B (AURKB Antibody Abstract)
Background Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body.  High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Synonyms: AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody
Gene ID 9212, 83776600
UniProt Q96GD4
Pathways TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Handling References for anti-Aurora Kinase B antibody (ABIN129612) Images back to top
Application Notes This affinity-purified antibody has been tested for use in ELISA and by western blot.  See below for specific protocol.  Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract.  HeLa cell lysate can be used as a positive control.
Restrictions For Research Use only

Handling

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details References for anti-Aurora Kinase B antibody (ABIN129612) Images back to top
Format Liquid
Concentration 1.21 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-Aurora Kinase B antibody (ABIN129612)

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Handling Images back to top
Product cited in:

Novais-Cruz, Alba Abad, van IJcken, Galjart, Jeyaprakash, Maiato, Ferrás: "Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription." in: eLife, Vol. 7, 2019

Xie, Lin, Wu, Tan, Cheng, Zhang: "Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway." in: Oncotarget, Vol. 9, Issue 17, pp. 13783-13795, 2018

Skowyra, Allan, Saurin, Clarke: "USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability." in: Cell reports, Vol. 23, Issue 3, pp. 852-865, 2018

Drpic, Almeida, Aguiar, Renda, Damas, Lewin, Larkin, Khodjakov, Maiato: "Chromosome Segregation Is Biased by Kinetochore Size." in: Current biology : CB, Vol. 28, Issue 9, pp. 1344-1356.e5, 2018

Shrestha, Conti, Tamura, Braun, Ramalingam, Cieslinski, Ries, Draviam: "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells." in: Nature communications, Vol. 8, Issue 1, pp. 150, 2017

Mo, Zhuang, Liu, Yao, Qin, Su, Zang, Wang, Zhang, Dou, Tian, Teng, Niu, Hill, Fang, Ding, Fu, Yao: "Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation." in: Nature chemical biology, Vol. 12, Issue 4, pp. 226-32, 2016

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." in: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, 2016

Asghar, Lajeunesse, Dulla, Combes, Thebault, Nigg, Elowe: "Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation." in: Nature communications, Vol. 6, pp. 8364, 2015

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." in: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, 2014

Carretero, Ruiz-Torres, Rodríguez-Corsino, Barthelemy, Losada: "Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres." in: The EMBO journal, Vol. 32, Issue 22, pp. 2938-49, 2014

Rivera, Ghenoiu, Rodríguez-Corsino, Mochida, Funabiki, Losada: "Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex." in: The EMBO journal, Vol. 31, Issue 6, pp. 1467-79, 2012

Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." in: The Journal of cell biology, Vol. 191, Issue 5, pp. 923-31, 2011

Images

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Handling References for anti-Aurora Kinase B antibody (ABIN129612) back to top
Supplier Images
Western Blotting (WB) image for anti-Aurora Kinase B (AURKB) (AA 223-234), (pThr232) antibody (ABIN129612) Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All...
Immunohistochemistry (IHC) image for anti-Aurora Kinase B (AURKB) (AA 223-234), (pThr232) antibody (ABIN129612) Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. Tissue: human placenta pH...