|Antigen||Aurora Kinase B (AURKB) Antibodies|
|Epitope||AA 227-238 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This Aurora Kinase B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
Product Details anti-Aurora Kinase B AntibodyTarget Details Aurora Kinase B Application Details Handling References for anti-Aurora Kinase B antibody (ABIN318827) Images
|Specificity||This antibody detects Aurora-B kinase (AurB).|
Species reactivity (expected):Rat, Bovine, Mouse, Chimpanzee, Dog, Pig.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.|
|Plasmids, Primers & others|
Target Details Aurora Kinase BProduct Details anti-Aurora Kinase B Antibody Application Details Handling References for anti-Aurora Kinase B antibody (ABIN318827) Images back to top
|Alternative Name||Aurora Kinase B (AURKB Antibody Abstract)|
|Background||Aurora-B kinase is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12|
|Pathways||TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Handling References for anti-Aurora Kinase B antibody (ABIN318827) Images back to top
ELISA: 1: 2000 - 1: 10000. Western Blot: 1: 250 - 1: 1000, detects a band of approximately 39 kDa in HeLa cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details References for anti-Aurora Kinase B antibody (ABIN318827) Images back to top
|Buffer||Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Aurora Kinase B antibody (ABIN318827)Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Handling Images back to top
Vader, Medema, Lens: "The chromosomal passenger complex: guiding Aurora-B through mitosis." in: The Journal of cell biology, Vol. 173, Issue 6, pp. 833-7, 2006
Keen, Taylor: "Aurora-kinase inhibitors as anticancer agents." in: Nature reviews. Cancer, Vol. 4, Issue 12, pp. 927-36, 2004