anti-Rat (Rattus) Aurora Kinase B antibody for Immunocytochemistry

Recommended Aurora Kinase B Antibody

Antigen
Aurora Kinase B (AURKB) Antibodies
  • AIK2
  • AIM-1
  • AIM1
  • ARK2
  • AurB
  • IPL1
  • PPP1R48
  • STK12
  • STK5
  • aurkb-sv1
  • aurkb-sv2
  • AIRK2
  • AL022959
  • Aik2
  • Aim1
  • Ark2
  • STK-1
  • Stk12
  • Stk5
  • Aur B
  • Aurora B
  • Aurora-B
  • CG6620
  • DmAIRK2
  • DmAurB
  • DmAuroraB
  • Dmel\\CG6620
  • IAL
  • IAL1
  • Ial
  • aurB
  • aurora B
  • ARK-2
  • XAIRK2
  • aik2
  • aim-1
  • aim1
  • airk2
  • ark2
  • ipl1
  • stk12
  • stk5
  • CHUNP6896
  • fa09g06
  • stka
  • wu:fa09g06
  • aurkb
  • AIRK2-B
  • XAIRK2-B
  • aurkb-a
  • aurkb-b
  • xAurora-B-B
  • aurora kinase B
  • aurora B
  • aurora kinase B S homeolog
  • AURKB
  • Aurkb
  • aurB
  • aurkb
  • aurkb.S
Epitope
pThr232
32
32
27
21
16
13
12
10
10
9
7
7
7
7
6
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
279
119
91
16
8
7
4
2
2
2
1
1
1
Host
Rabbit
215
60
7
7
Clonality
Polyclonal
Conjugate
This Aurora Kinase B antibody is un-conjugated
12
9
9
8
8
7
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
229
142
96
92
43
32
15
11
11
10
8
2
2
1
Options

Available images

Catalog No. ABIN6256516
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13 ABIN6260155 ELISA ICC IF WB Rabbit IgG Polyclonal 0
12.446655 ABIN151760 ICC IF IHC IHC (p) IP MA WB Rabbit C-Term Polyclonal 12
10 ABIN6260154 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
1 ABIN6582341 ICC IF IHC WB Rabbit pThr232 Polyclonal 0
1 ABIN6583165 ICC IF WB Rabbit Polyclonal 0
-4.0533447 ABIN4282455 ICC IF WB Mouse IgG2a 3F11 0
-4.0533447 ABIN4282457 ICC IF WB Mouse IgG1 6G2 0
-13.053345 ABIN4282454 ICC IF WB Mouse IgG2a 3F11 0
-13.053345 ABIN4282456 ICC IF WB Mouse IgG1 6G2 0
-13.053345 ABIN4282460 ICC IF WB Alexa Fluor 647 Mouse IgG2a 3F11 0
-13.053345 ABIN4282458 ICC IF WB Alexa Fluor 405 Mouse IgG2a 3F11 0
-13.053345 ABIN4282461 ICC IF WB Alexa Fluor 700 Mouse IgG2a 3F11 0
-13.053345 ABIN4282463 ICC IF WB Biotin Mouse IgG2a 3F11 0
-13.053345 ABIN4282464 ICC IF WB DyLight 650 Mouse IgG2a 3F11 0
-13.053345 ABIN4282465 ICC IF FITC Mouse IgG2a 3F11 0
-13.053345 ABIN4282466 ICC IF WB DyLight 680 Mouse IgG2a 3F11 0
-13.053345 ABIN4282467 ICC IF WB DyLight 488 Mouse IgG2a 3F11 0
-13.053345 ABIN4282468 ICC IF WB DyLight 755 Mouse IgG2a 3F11 0
-13.053345 ABIN4282472 ICC IF WB DyLight 350 Mouse IgG2a 3F11 0
-13.053345 ABIN4282459 ICC IF WB Alexa Fluor 488 Mouse IgG2a 3F11 0

General

Antigen Aurora Kinase B (AURKB) Antibodies
Epitope pThr232
(32), (32), (27), (21), (16), (13), (12), (10), (10), (9), (7), (7), (7), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(279), (119), (91), (16), (8), (7), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(215), (60), (7), (7)
Clonality
Polyclonal
Conjugate This Aurora Kinase B antibody is un-conjugated
(12), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(229), (142), (96), (92), (43), (32), (15), (11), (11), (10), (8), (2), (2), (1)
Supplier

Product Details anti-Aurora Kinase B Antibody

Target Details Aurora Kinase B Application Details Handling Images
Specificity Phospho-AurB (Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Threonine 232
Cross-Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human AurB around the phosphorylation site of Threonine 232
Isotype IgG
Plasmids, Primers & others

Target Details Aurora Kinase B

Product Details anti-Aurora Kinase B Antibody Application Details Handling Images back to top
Antigen
Alternative Name AurB (AURKB Antibody Abstract)
Background

Description: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Gene: AURKB

Molecular Weight 39kDa
Gene ID 9212
UniProt Q96GD4
Pathways TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) Western blot analysis of AurB phosphorylation expression in Nocodazole treated COS7 w...
Immunohistochemistry (IHC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 at 1/100 staining human Bone marrow tissue sections by IHC-P. The tissue ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 staining HT-1080 cells by ICC/IF. Cells were fixed with PFA and permeabil...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Aurora Kinase B (AURKB) (pThr232) antibody (ABIN6256516) ABIN6267684 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized...