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|Antigen||Caspase Recruitment Domain Family, Member 11 (CARD11) Antibodies|
|Conjugate||This CARD11 antibody is un-conjugated Alternatives|
|Method Type||Peptide ELISA|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CARD11 AntibodyTarget Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN184957) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN369694|
|Cross-Reactivity||Mouse (Murine), Dog (Canine), Cow (Bovine)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.|
Target Details CARD11Product Details anti-CARD11 Antibody Application Details Handling References for anti-CARD11 antibody (ABIN184957) Images back to top
|Alternative Name||CARD11 (CARD11 Antibody Abstract)|
|Background||Synonyms: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3|
|Gene ID||84433, 108723|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-CARD11 Antibody Target Details CARD11 Handling References for anti-CARD11 antibody (ABIN184957) Images back to top
ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150+65 kDa bands observed in lysates of cell line Jurkat (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5 µg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CARD11 Antibody Target Details CARD11 Application Details References for anti-CARD11 antibody (ABIN184957) Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
References for anti-CARD11 antibody (ABIN184957)Product Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling Images back to top
Bertin, Wang, Guo, Jacobson, Poyet, Srinivasula, Merriam, DiStefano, Alnemri: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001
ImagesProduct Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN184957) back to top