anti-Human CARD11 antibody for ELISA

Recommended CARD11 Antibody (supplied by: Log in to see )

Antigen
Caspase Recruitment Domain Family, Member 11 (CARD11) Antibodies
  • BENTA
  • BIMP3
  • CARMA1
  • PPBL
  • 0610008L17Rik
  • 2410011D02Rik
  • CARD11
  • LOC100232755
  • caspase recruitment domain family member 11
  • caspase recruitment domain family, member 11
  • caspase recruitment domain-containing protein 11
  • CARD11
  • Card11
  • LOC100550812
  • card11
Epitope
C-Term
26
10
5
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
82
47
12
5
5
1
1
1
1
Host
Goat
62
20
1
1
Clonality
Polyclonal
Conjugate
This CARD11 antibody is un-conjugated
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
77
48
47
20
11
5
4
3
3
2
1
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Catalog No. ABIN184957
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1534305 ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal 1
1 ABIN249816 IHC (p) IHC ELISA WB Goat C-Term Log in to see Polyclonal 2
1 ABIN364421 ELISA IHC IHC (p) WB Goat AA 1160-1171 Log in to see Polyclonal 0
1 ABIN3183648 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2889893 ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal 0
1 ABIN6268186 ELISA WB Rabbit IgG pSer652 Log in to see Polyclonal 0
1 ABIN2465495 IHC ELISA WB Goat C-Term Log in to see Polyclonal 0
1 ABIN4287879 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN6280384 ELISA IF IHC (p) WB Rabbit IgG AA 10-90, N-Term Log in to see Polyclonal 0
1 ABIN1732635 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal 0
1 ABIN6268602 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6260459 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1030313 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN229655 ELISA WB Rabbit IgG Thr175 Log in to see 0
1 ABIN566564 ELISA WB Mouse AA 481-580, partial Log in to see Polyclonal 0
1 ABIN5859673 ELISA IHC WB Goat Log in to see Polyclonal 0
1 ABIN206030 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2561564 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN4287878 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2218567 IHC ELISA WB Alkaline Phosphatase (AP) Goat C-Term Log in to see Polyclonal 0

General

Antigen Caspase Recruitment Domain Family, Member 11 (CARD11) Antibodies
Epitope C-Term
(26), (10), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(82), (47), (12), (5), (5), (1), (1), (1), (1)
Host Goat
(62), (20), (1), (1)
Clonality
Polyclonal
Conjugate This CARD11 antibody is un-conjugated
(1), (1), (1), (1), (1), (1)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(77), (48), (47), (20), (11), (5), (4), (3), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-CARD11 Antibody

Target Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN184957) Images
Blocking Peptide Blocking peptide for this product available: ABIN369694
Cross-Reactivity Mouse (Murine), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.
Plasmids, Primers & others

Target Details CARD11

Product Details anti-CARD11 Antibody Application Details Handling References for anti-CARD11 antibody (ABIN184957) Images back to top
Antigen
Alternative Name CARD11 (CARD11 Antibody Abstract)
Background Synonyms: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Gene ID 84433, 108723
NCBI Accession NP_115791
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-CARD11 Antibody Target Details CARD11 Handling References for anti-CARD11 antibody (ABIN184957) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150+65 kDa bands observed in lysates of cell line Jurkat (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details References for anti-CARD11 antibody (ABIN184957) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-CARD11 antibody (ABIN184957)

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling Images back to top
Background publications

Bertin, Wang, Guo, Jacobson, Poyet, Srinivasula, Merriam, DiStefano, Alnemri: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001

Images

Product Details anti-CARD11 Antibody Target Details CARD11 Application Details Handling References for anti-CARD11 antibody (ABIN184957) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (ABIN184957) ABIN184957 staining (1µg/ml) of human Jurkat cells (RIPA buffer, 35µg total protein p...