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|Antigen||Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This CBL antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-CBL AntibodyTarget Details CBL Application Details Handling Images
|Specificity||This antibody detects RNF55 / CBL at C-term.|
Species reactivity (expected):Human, Rat, Dog, Pig, Cow.
Species reactivity (tested):Mouse.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from C Terminus of the protein sequence according to NP_005179.2 Genename: CBL|
Target Details CBLProduct Details anti-CBL Antibody Application Details Handling Images back to top
|Alternative Name||CBL / RNF55 (CBL Antibody Abstract)|
|Background||Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias, c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4,11) or t(11,14) abnormalities.Synonyms: CBL2, Casitas B-lineage lymphoma proto-oncogene, E3 ubiquitin-protein ligase CBL, Proto-oncogene c-CBL, RING finger protein 55, Signal transduction protein CBL, c-CBL|
|Pathways||TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-CBL Antibody Target Details CBL Handling Images back to top
Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 120 kDa band seen in 3T3 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CBL Antibody Target Details CBL Application Details Images back to top
|Buffer||Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|