anti-Human CD247 antibody for Functional Studies

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • CD3-ZETA
  • CD3H
  • CD3Q
  • CD3Z
  • T3Z
  • TCRZ
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • TCRzeta
  • CD247 molecule
  • CD247 antigen
  • CD3zeta-2
  • CD3zeta-1
  • Cd247 molecule
  • CD247
  • Cd247
  • cd3z
  • cd247
Epitope
Cytoplasmic Domain
38
21
14
10
9
9
7
7
6
5
5
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
223
149
35
7
4
4
1
1
1
1
Host
Mouse
101
101
20
15
12
5
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
19
11
10
8
7
6
6
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
Functional Studies (Func), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
191
145
97
37
35
30
18
18
5
4
4
3
3
1
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Catalog No. ABIN480375
$ 903.83
Plus shipping costs $45.00

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope Cytoplasmic Domain
(38), (21), (14), (10), (9), (9), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(223), (149), (35), (7), (4), (4), (1), (1), (1), (1)
Host Mouse
(101), (101), (20), (15), (12), (5)
Clonality
Monoclonal
Conjugate This CD247 antibody is un-conjugated
(19), (11), (10), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(191), (145), (97), (37), (35), (30), (18), (18), (5), (4), (4), (3), (3), (1)
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling Images
Specificity Directed against human TCR z, however, it is also reactive with mouse TCR z. Recognizes both the un-phosphorylated and phosphorylated forms of TCR z. Does not cross-react with the B Cell protein tyrosine kinase Syk. Reactivity has been confirmed by Western blot analysis of cell lysates from human Jurkat T cells. Additionally, this antibody is capable of co-precipitating the z chain and associated tyrosine phosphorylated protein ZAP-70. ZAP-70 is a protein tyrosine kinase which interacts with the TCR z chain via two SH2 domains located in the ZAP-70 protein.
Purification Protein A purified
Immunogen A synthetic peptide corresponding to the cytoplasmic domain of the human T Cell Receptor z (zeta) chain coupled to a protein carrier.

Type of Immunogen: Synthetic peptide
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD247 / CD3 Zeta (CD247 Antibody Abstract)
Background Name/Gene ID: CD247

Synonyms: CD247, CD3 Zeta, CD3H, CD3Z, CD247 antigen, zeta subunit, CD247 Molecule, CD3zeta chain, T3Z, TCR zeta chain, TCRZ, T-cell receptor T3 zeta chain, CD247 antigen, CD3-ZETA, CD3Q
Gene ID 919
UniProt P20963
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling Images back to top
Application Notes Approved: ELISA (0.1 - 1 μg/mL), Func, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1 μg/mL. Immunoprecipitation/ IP-Kinase Assay: 5 μg. Western Blot: 1-3 μg/mL Note: To co-IP TCR z and ZAP70, prepare Jurkat cell lysates using 1 % NP-40.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.