anti-Mouse (Murine) CD247 antibody for Flow Cytometry

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • CD3-ZETA
  • CD3H
  • CD3Q
  • CD3Z
  • T3Z
  • TCRZ
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • TCRzeta
  • CD247 molecule
  • CD247 antigen
  • CD3zeta-2
  • CD3zeta-1
  • Cd247 molecule
  • CD247
  • Cd247
  • cd3z
  • cd247
Epitope
pTyr72
38
21
14
10
9
9
7
7
6
5
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
224
149
37
7
4
4
1
1
1
1
Host
Mouse
105
99
19
15
12
5
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
17
11
10
8
7
6
6
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
196
143
100
38
36
29
19
17
5
4
3
3
2
1
Options
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Catalog No. ABIN2749060
$ 343.07
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16 ABIN4900705 CyTOF FACS ICC IHC IP Rat IgG2b Log in to see 17A2 7
15.434076 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17 0
15.434076 ABIN2749058 FACS WB Mouse IgG1 pTyr142 Log in to see EM-54 0
15.434076 ABIN2749059 FACS WB Mouse IgG1 pTyr111, pTyr123 Log in to see EM-55 0
14.358341 ABIN4898316 FACS PE Rat IgG2b Log in to see 17A2 2
14.358341 ABIN4898308 FACS PerCP Rat IgG2b Log in to see 17A2 1
13 ABIN4900709 CyTOF FACS IP Hamster IgG Log in to see 145-2C11 8
12.858341 ABIN4898312 FACS ICC Alexa Fluor 488 Rat IgG2b Log in to see 17A2 0
12.858341 ABIN4898321 FACS ICC Alexa Fluor 594 Rat IgG2b Log in to see 17A2 0
12.858341 ABIN4900655 FACS ICC IHC IP Biotin Rat IgG2b Log in to see 17A2 0
12.434076 ABIN161390 FACS ELISA WB FITC Hamster IgG Log in to see H146-968 0
12.434076 ABIN161389 FACS ELISA WB FITC Hamster IgG Log in to see H146-968 0
12.434076 ABIN161388 FACS ELISA WB Biotin Hamster IgG Log in to see H146-968 0
12.434076 ABIN161387 FACS ELISA WB Biotin Hamster IgG Log in to see H146-968 0
9.858341 ABIN4898310 FACS Fluorescein Rat IgG2b Log in to see 17A2 0
9.858341 ABIN4898306 FACS APC Rat IgG2b Log in to see 17A2 0
9.858341 ABIN4898314 FACS Alexa Fluor 700 Rat IgG2b Log in to see 17A2 0
9.858341 ABIN4898318 FACS Alexa Fluor 647 Rat IgG2b Log in to see 17A2 0
9.858341 ABIN4898322 FACS Alexa Fluor 405 Rat IgG2b Log in to see 17A2 0
9.858341 ABIN4898319 FACS Alexa Fluor 750 Rat IgG2b Log in to see 17A2 0

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope pTyr72
(38), (21), (14), (10), (9), (9), (7), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(224), (149), (37), (7), (4), (4), (1), (1), (1), (1)
Host Mouse
(105), (99), (19), (15), (12), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(17), (11), (10), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(196), (143), (100), (38), (36), (29), (19), (17), (5), (4), (3), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN2749060) Images
Specificity The mouse monoclonal antibody EM-26 recognizes phosphorylated tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Cross-Reactivity (Details) Human, Mouse
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
Clone EM-26
Isotype IgG2b
Plasmids, Primers & others

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 antibody (ABIN2749060) Images back to top
Antigen
Alternative Name CD3 zeta (CD247 Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 antibody (ABIN2749060) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 antibody (ABIN2749060) Images back to top
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD247 antibody (ABIN2749060)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Dopfer, Schöpf, Louis-Dit-Sully, Dengler, Höhne, Klescová, Prouza, Suchanek, Reth, Schamel: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, 2010

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 antibody (ABIN2749060) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 Molecule (CD247) (pTyr72) antibody (ABIN2749060) Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17...
Immunofluorescence (IF) image for anti-CD247 Molecule (CD247) (pTyr72) antibody (ABIN2749060) Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed b...