anti-Human CD28 antibody for ELISA

Recommended CD28 Antibody

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Epitope
Internal Region
19
17
16
14
11
11
7
7
6
5
4
3
3
3
2
1
1
1
1
1
1
Reactivity
Human
420
320
86
45
35
27
27
16
12
7
6
2
2
2
1
1
Host
Goat
383
165
115
29
29
26
7
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
89
80
57
45
14
14
10
8
8
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
593
151
147
131
95
77
50
48
36
26
24
13
9
4
3
2
2
1
1
1
1
Options

Available images

Catalog No. ABIN625822
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
16.47682 ABIN3187775 ELISA WB Rabbit IgG Internal Region Polyclonal 0
13.47682 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Polyclonal 0
13.47682 ABIN6941132 Coat ELISA Mouse IgG1, kappa C28-77 0
13.47682 ABIN6941135 Coat ELISA Mouse IgG1, kappa C28-74 0
13.47682 ABIN6936659 Coat ELISA Mouse IgG1, kappa C28-77 0
13.47682 ABIN6936662 Coat ELISA Mouse IgG1, kappa C28-74 0
13.47682 ABIN2136363 ELISA WB Rabbit IgG pTyr218 Polyclonal 0
10 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Polyclonal 0
10 ABIN5860667 ELISA Goat Polyclonal 0
7 ABIN6255852 ELISA ICC IF WB Rabbit IgG pTyr218 Polyclonal 0
4 ABIN2464509 ELISA WB Goat Internal Region Polyclonal 0
4 ABIN1531543 ELISA WB Rabbit IgG AA 171-220, pTyr218 Polyclonal 0
4 ABIN793286 ELISA WB Goat AA 73-87 Polyclonal 0
4 ABIN2662242 ELISA Pacific Blue Mouse IgG1 kappa CD28-2 9
4 ABIN2659213 ELISA PE-Cy7 Mouse IgG1 kappa CD28-2 9
4 ABIN5956125 ELISA WB Rabbit IgG Internal Region Polyclonal 0
4 ABIN2691620 ELISA FACS Rabbit IgG AA 1-152 007 0
4 ABIN5574300 ELISA WB Goat Polyclonal 0
4 ABIN2752138 ELISA WB Mouse IgG1 kappa AA 18-152 8D7 0
4 ABIN2752133 ELISA WB Mouse IgG1 kappa AA 18-152 1H3 0

General

Antigen CD28 Antibodies
Epitope Internal Region
(19), (17), (16), (14), (11), (11), (7), (7), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(420), (320), (86), (45), (35), (27), (27), (16), (12), (7), (6), (2), (2), (2), (1), (1)
Host Goat
(383), (165), (115), (29), (29), (26), (7), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(89), (80), (57), (45), (14), (14), (10), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(593), (151), (147), (131), (95), (77), (50), (48), (36), (26), (24), (13), (9), (4), (3), (2), (2), (1), (1), (1), (1)
Supplier

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Purpose CD28
Blocking Peptide Blocking peptide for this product available: ABIN2815357
Sequence SQQLQVYSKT GFNCD
Specificity The immunizing peptide corresponds to a part of the extracellular domain.
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen SQQLQVYSKTGFNCD

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28, CD28 Molecule, MGC138290, Tp44, CD28 antigen, CD28 antigen (Tp44), T-cell-specific surface glycoprotein
Gene ID 940
NCBI Accession NP_006130
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes

DS WB Results: Approx 48 kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1 kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD28 (CD28) (Internal Region) antibody (ABIN625822) Antibody (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen ...
 image for anti-CD28 (CD28) (Internal Region) antibody (ABIN625822) Antibody Negative Control showing staining of paraffin embedded Human Lymph Node, wit...