anti-Human CD28 antibody for Immunofluorescence

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Epitope
AA 182-208, C-Term
17
16
14
12
11
11
10
10
7
7
6
4
3
3
3
3
3
3
3
1
1
1
1
1
1
Reactivity
Human
600
446
100
70
67
60
60
43
34
27
16
7
2
2
2
2
1
1
1
Host
Rabbit
625
189
98
70
59
31
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
119
97
79
63
26
22
20
19
18
16
12
12
12
12
12
12
11
10
10
9
9
8
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
903
262
199
193
155
147
145
129
83
70
48
26
13
13
9
8
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.099838 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal 0
1 ABIN2665915 FACS IF Mouse IgG1 kappa Log in to see CD28-2 10
1 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2 0
1 ABIN5530792 IF WB Rabbit Ig Fraction AA 182-208, C-Term Log in to see Polyclonal 0
1 ABIN5574294 FACS IF Mouse IgG1 full length Log in to see C28-1636 1
1 ABIN6158507 FACS IF CF®405S Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158515 FACS IF CF®647 Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158523 FACS IF CF®770 Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158529 FACS IF CF®488A Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158553 FACS IF CF®647 Mouse IgG1 Log in to see C28-1636 0
1 ABIN6158563 FACS IF CF®680 Mouse IgG1 Log in to see C28-1636 0
1 ABIN6158517 FACS IF CF®555 Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158527 FACS IF CF®680R Mouse IgG2a, kappa Log in to see 204-12 0
1 ABIN6158551 FACS IF CF®543 Mouse IgG1 Log in to see C28-1636 0
1 ABIN6158595 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see C28-75 0
1 ABIN6158587 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see C28-75 0
1 ABIN6158623 FACS IF IHC (f) CF405M Mouse IgG1, kappa Log in to see C28-77 0
1 ABIN6158591 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see C28-75 0
1 ABIN6158659 FACS IF CF®405S Mouse IgG1, kappa Log in to see CB28 0
1 ABIN6158671 FACS IF CF®660R Mouse IgG1, kappa Log in to see CB28 0

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(17), (16), (14), (12), (11), (11), (10), (10), (7), (7), (6), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Reactivity Human
(600), (446), (100), (70), (67), (60), (60), (43), (34), (27), (16), (7), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(625), (189), (98), (70), (59), (31), (12), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(119), (97), (79), (63), (26), (22), (20), (19), (18), (16), (12), (12), (12), (12), (12), (12), (11), (10), (10), (9), (9), (8), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(903), (262), (199), (193), (155), (147), (145), (129), (83), (70), (48), (26), (13), (13), (9), (8), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig Fraction
Plasmids, Primers & others

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (AA 182-208), (C-Term) antibody (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 (CD28) (AA 182-208), (C-Term) antibody (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...