anti-Human CD28 antibody for Immunofluorescence

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 182-208, C-Term
16
15
15
11
11
11
11
10
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
1
Reactivity
Human
495
422
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Rabbit
527
203
91
77
40
30
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
113
95
74
38
22
21
20
20
18
18
17
13
13
13
13
12
12
12
8
8
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
795
211
185
159
143
141
133
130
89
77
26
21
13
9
8
6
6
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.911091 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
1 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN2665916 FACS IF Mouse IgG1 kappa Log in to see CD28-2 8
1 ABIN2665915 FACS IF Mouse IgG1 kappa Log in to see CD28-2 10
1 ABIN4291200 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN5530792 IF WB Rabbit Ig Fraction AA 182-208, C-Term Log in to see Polyclonal
1 ABIN2592986 ELISA IF WB Rabbit IgG AA 188-217 Log in to see Polyclonal
1 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN4291333 FACS IF PE Mouse IgG2a kappa Log in to see 204-12
1 ABIN4291249 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75
1 ABIN4291247 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75
1 ABIN4291250 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77
1 ABIN4291251 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77
1 ABIN4291252 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see C28-77
1 ABIN4291254 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28
1 ABIN4291248 FACS ICC IF IHC (p) Mouse IgG1 kappa Log in to see C28-75
1 ABIN4291255 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28
1 ABIN4291253 FACS ICC IF IHC (fro) Mouse IgG1 kappa Log in to see CB28
1 ABIN1966662 ELISA ICC IF WB Biotin Rabbit IgG AA 182-208 Log in to see Polyclonal
1 ABIN1964045 ELISA ICC IF WB APC Rabbit IgG AA 182-208 Log in to see Polyclonal

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(16), (15), (15), (11), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Reactivity Human
(495), (422), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(527), (203), (91), (77), (40), (30), (12), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(113), (95), (74), (38), (22), (21), (20), (20), (18), (18), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(795), (211), (185), (159), (143), (141), (133), (130), (89), (77), (26), (21), (13), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (AA 182-208), (C-Term) antibody (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 (CD28) (AA 182-208), (C-Term) antibody (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...