anti-Mouse (Murine) CD28 antibody for Cytotoxicity Test

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Reactivity
Mouse (Murine)
595
440
96
70
67
61
61
44
34
27
16
7
2
2
2
2
1
1
1
Host
Golden Syrian Hamster
617
187
98
66
58
31
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
116
95
77
62
26
22
19
18
17
15
12
12
12
12
12
12
11
10
10
9
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
Application
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
889
267
193
191
156
148
144
129
85
70
48
26
13
12
9
8
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2689144
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176978 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Log in to see 37-51 14
1 ABIN2689145 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Log in to see 37-51 0

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(595), (440), (96), (70), (67), (61), (61), (44), (34), (27), (16), (7), (2), (2), (2), (2), (1), (1), (1)
Host Golden Syrian Hamster
(617), (187), (98), (66), (58), (31), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(116), (95), (77), (62), (26), (22), (19), (18), (17), (15), (12), (12), (12), (12), (12), (12), (11), (10), (10), (9), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(889), (267), (193), (191), (156), (148), (144), (129), (85), (70), (48), (26), (13), (12), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN2689144) Images
Brand BD Pharmingen™
Characteristics The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of CD28 expression on splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb [Mouse BD Fc Block™] (Cat. No. 553141), then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046) and FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030) in addition to staining with purified anti-mouse CD28 clone 37.51 (right panel). Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 (Cat. No. 554029), followed by streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD28 - Purified - Clone 37.51 - Isotype Syrian Hamster IgG2, λ1 - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse EL-4 (T-cell lymphoma) Cells
Clone 37-51
Isotype IgG2, lambda
Plasmids, Primers & others

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN2689144) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN2689144) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN2689144) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD28 antibody (ABIN2689144)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, 1997

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, 1993

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, 1993

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992