anti-Mouse (Murine) CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Epitope
AA 171-220, pTyr218
16
16
15
12
11
11
11
9
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
492
413
101
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Rabbit
524
203
91
68
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
112
95
73
56
21
20
20
19
17
17
13
13
13
13
12
12
12
8
8
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
ELISA, Western Blotting (WB)
783
209
185
161
145
143
133
129
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN1531543
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2464553 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN793287 ELISA WB Goat AA 137-149, Extracellular Domain Log in to see Polyclonal 0
1 ABIN2000361 ELISA IHC (p) Rabbit IgG AA 1-149 Log in to see Polyclonal 1
1 ABIN1584203 ELISA FACS IHC WB Rabbit N-Term Log in to see Polyclonal 1
1 ABIN5539489 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal 0
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal 0
1 ABIN2883595 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal 0
1 ABIN226843 FACS ELISA Hamster IgG Log in to see 0
1 ABIN2885744 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal 0
1 ABIN2592985 ELISA WB Rabbit IgG AA 33-174 Log in to see Polyclonal 0
1 ABIN2224853 ELISA WB Biotin Goat Log in to see Polyclonal 0
1 ABIN2224854 ELISA WB FITC Goat Log in to see Polyclonal 0
1 ABIN2224856 ELISA WB PE Goat Log in to see Polyclonal 0
1 ABIN2224852 ELISA WB APC Goat Log in to see Polyclonal 0
1 ABIN2224851 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal 0
1 ABIN2213288 ELISA Rabbit IgG AA 1-149 Log in to see Polyclonal 0
1 ABIN2561705 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN2224807 ELISA WB Goat Log in to see Polyclonal 0
1 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal 0

General

Antigen CD28 Antibodies
Epitope AA 171-220, pTyr218
(16), (16), (15), (12), (11), (11), (11), (9), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(492), (413), (101), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(524), (203), (91), (68), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(112), (95), (73), (56), (21), (20), (20), (19), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Western Blotting (WB)
(783), (209), (185), (161), (145), (143), (133), (129), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity

CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.

PhosphorylationH:Y218 M:Y216

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background

Synonyms: SCFR, Proto-oncogene tyrosine-protein kinase Kit, c-kit, CD117 antigen

NCBI Gene Symbol: CD28

Molecular Weight 25 kDa
Gene ID 940
OMIM 186760
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes WB: 1:500~1:1000 ELISA: 1:40000
Comment

Unigene-Number: Hs.591629 (NCBI Gene Symbol: CD28)

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (AA 171-220), (pTyr218) antibody (ABIN1531543) Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h...