anti-Mouse (Murine) CD28 antibody for Immunocytochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Epitope
AA 33-174
16
16
15
12
11
11
11
10
8
7
6
4
4
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
496
413
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Rabbit
527
203
91
70
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
113
96
74
56
21
20
20
19
17
17
13
13
13
13
12
12
12
8
8
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
788
210
185
160
143
140
132
128
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2118849
$ 324.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN457400 Func ICC FACS IHC (fro) IP Hamster IgG Extracellular Domain Log in to see 37-51 10
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN440723 CyTOF FACS Func ICC IF IHC IHC (fro) IP Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957771 FACS ICC IF IHC IHC (fro) Alexa Fluor 405 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957772 FACS ICC IF IHC IHC (fro) Alexa Fluor 488 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957773 FACS ICC IF IHC IHC (fro) Alexa Fluor 647 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957774 FACS ICC IF IHC IHC (fro) Alexa Fluor 700 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957776 ICC IF IHC IHC (fro) Biotin Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957777 FACS ICC IF IHC IHC (fro) DyLight 650 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957778 FACS ICC IF IHC IHC (fro) FITC Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957779 FACS ICC IF IHC IHC (fro) DyLight 680 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957780 FACS ICC IF IHC IHC (fro) DyLight 488 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957782 FACS ICC IF IHC IHC (fro) DyLight 755 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957783 FACS ICC IF IHC (fro) PerCP Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957785 FACS ICC IF IHC IHC (fro) DyLight 550 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957786 FACS ICC IF IHC IHC (fro) DyLight 350 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN4957787 FACS ICC IF IHC IHC (fro) DyLight 405 Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN3199703 FACS Func ICC IHC (fro) IP Golden Syrian Hamster IgG Log in to see 37-51
1 ABIN2962182 Func ICC IHC (fro) FACS IHC (p) WB Golden Syrian Hamster IgG Log in to see (37-51)

General

Antigen CD28 Antibodies
Epitope AA 33-174
(16), (16), (15), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(496), (413), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(527), (203), (91), (70), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(113), (96), (74), (56), (21), (20), (20), (19), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(788), (210), (185), (160), (143), (140), (132), (128), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against CD28. It has been selected for its ability to recognize CD28 in immunohistochemical staining and western blotting.
Characteristics The antibody is a rabbit polyclonal antibody raised against CD28. It has been selected for its ability to recognize CD28 in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen CD28 (Asp33-Val174)
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cluster Of Differentiation 28 (CD28) (CD28 Antibody Abstract)
Background Alternative Names: Tp44, T-Cell-Specific Surface Glycoprotein
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment Store at 4 °C for frequent use. Stored at -20 °C to -80 °C in a manual defrost freezer for one year without detectable loss of activity.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (AA 33-174) antibody (ABIN2118849) anti-CD28 (CD28) (AA 33-174) antibody
Western Blotting (WB) image for anti-CD28 (CD28) (AA 33-174) antibody (ABIN2118849) Western Blot; Sample: Mouse Lymph node lysate; Primary Ab: 5µg/ml Rabbit Anti-Mouse C...
 image for anti-CD28 (CD28) (AA 33-174) antibody (ABIN2118849) Used in DAB staining on fromalin fixed paraffin- embedded brain tissue
 image for anti-CD28 (CD28) (AA 33-174) antibody (ABIN2118849) DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue