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|Reactivity||Human, Non-Human Primate Alternatives|
|Conjugate||This CD28 antibody is conjugated to PerCP Alternatives|
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN457388) Images
The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
HLDA V., WS Code 5T CD28.05
|Cross-Reactivity (Details)||Human, Non-Human Primates|
|Immunogen||DC22.214.171.124 murine T cell hybridoma transfected with human CD28 cDNA|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN457388) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN457388) Images back to top
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.|
The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN457388) Images back to top
|Reconstitution||No reconstitution is necessary.|
|Buffer||The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Do not freeze.
Avoid prolonged exposure to light.
|Storage Comment||Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.|
References for anti-CD28 antibody (ABIN457388)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
|Product cited in:||
Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004
Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004
Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001
Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999
Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999
Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993
ImagesProduct Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN457388) back to top