anti-Rat (Rattus) CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Reactivity
Rat (Rattus)
494
414
100
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Mouse
524
203
92
68
40
29
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is conjugated to FITC
112
94
73
56
21
20
20
19
17
17
13
13
13
13
12
12
12
8
8
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
783
208
185
161
146
144
133
129
88
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2749055
$ 290.95
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2749056 Func FACS IP PE Mouse IgG1 Log in to see JJ319 8
1 ABIN611634 Func FACS IP Mouse IgG1 Log in to see JJ319 9
1 ABIN120439 Func FACS IP Mouse IgG1 Log in to see JJ319 2
1 ABIN593899 Func FACS IP Mouse IgG1 Log in to see JJ319 0
1 ABIN1967871 Func FACS IP Mouse IgG1 Log in to see JJ319 0
1 ABIN3199701 FACS Func IP FITC Mouse IgG1 Log in to see JJ319 0
1 ABIN3199700 FACS Func IP Mouse IgG1 Log in to see JJ319 0
1 ABIN3199702 FACS Func IP PE Mouse IgG1 Log in to see JJ319 0
1 ABIN217615 Func FACS IP Mouse IgG1 Log in to see 0
1 ABIN2567439 Func FACS Mouse IgG1 Log in to see JJ319 0
1 ABIN2567440 Func FACS Mouse IgG1 Log in to see JJ319 0
1 ABIN5574299 FACS Func IP Mouse IgG1 Log in to see JJ319 0
1 ABIN3037034 Func FACS IP Mouse IgG1 Log in to see 0
1 ABIN3037036 Func FACS IP PE Mouse IgG1 Log in to see 0
1 ABIN3037035 Func FACS IP FITC Mouse IgG1 Log in to see 0

General

Antigen CD28 Antibodies
Reactivity Rat (Rattus)
(494), (414), (100), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(524), (203), (92), (68), (40), (29), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is conjugated to FITC
(112), (94), (73), (56), (21), (20), (20), (19), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(783), (208), (185), (161), (146), (144), (133), (129), (88), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN2749055) Images
Specificity The mouse monoclonal antibody JJ319 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Immunogen Mouse A20J B lymphoma cells transfected with rat CD28
Clone JJ319
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN2749055) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. Its analog CTLA-4, expressed on the surface of activated T cells, has higher affinity to CD80 and CD86 than CD28 and downregulates T cell activity.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN2749055) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN2749055) Images back to top
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD28 antibody (ABIN2749055)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Guillonneau, Séveno, Dugast, Li, Renaudin, Haspot, Usal, Veziers, Anegon, Vanhove: "Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8164-71, 2007

Kerstan, Armbruster, Leverkus, Hünig: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, 2006

van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, 2004

Lühder, Huang, Dennehy, Guntermann, Müller, Winkler, Kerkau, Ikemizu, Davis, Hanke, Hünig: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, 2003

Laskowski, Pratschke, Wilhelm, Dong, Beato, Taal, Gasser, Hancock, Sayegh, Tilney: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, 2002

Haspot, Villemain, Laflamme, Coulon, Olive, Tiollier, Soulillou, Vanhove: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, 2002

Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, 2001

Dengler, Szabo, Sido, Nottmeyer, Zimmerman, Vahl, Hünig, Meuer: "Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation." in: Transplantation, Vol. 67, Issue 3, pp. 392-8, 1999

Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, 1997