anti-Sheep (Ovine) CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Sheep (Ovine)
600
445
100
70
67
59
59
42
34
27
16
7
2
2
2
2
1
1
1
Host
Mouse
624
189
99
70
59
31
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
119
97
79
63
26
22
20
19
18
16
12
12
12
12
12
12
11
10
10
9
9
8
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunofluorescence (IF)
902
262
199
193
155
148
145
129
82
70
48
26
13
13
9
8
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN3025652
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3025653 Func FACS IF Mouse IgG2a, kappa Log in to see 204-12 0

General

Antigen CD28 Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Sheep (Ovine)
(600), (445), (100), (70), (67), (59), (59), (42), (34), (27), (16), (7), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(624), (189), (99), (70), (59), (31), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(119), (97), (79), (63), (26), (22), (20), (19), (18), (16), (12), (12), (12), (12), (12), (12), (11), (10), (10), (9), (9), (8), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunofluorescence (IF)
(902), (262), (199), (193), (155), (148), (145), (129), (82), (70), (48), (26), (13), (13), (9), (8), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
Clone 204-12
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Recognizes a glycoprotein of 44-88  kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Optimal dilution of the CD28 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Functional Activity (Order SAF formulation)
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).