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|Antigen||CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies|
|Epitope||AA 1-105 Alternatives|
|Conjugate||This CD3D antibody is un-conjugated Alternatives|
ELISA, ELISA (Detection), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CD3D AntibodyTarget Details CD3D Application Details Handling Images
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA
|Characteristics||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3D (rh CD3D, ABIN2003686 NP_000723.1, Met 1-Ala 105,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
|Purification||purified by Protein A affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human CD3D protein (ABIN2003686)|
Target Details CD3DProduct Details anti-CD3D Antibody Application Details Handling Images back to top
|Alternative Name||CD3D (CD3D Antibody Abstract)|
|Background||T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T- /B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.|
|Pathways||TCR Signaling, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD3D Antibody Target Details CD3D Handling Images back to top
WB: 5-10 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD3D. The detection limit for Human CD3D is 0.039 ng/well.
ELISA(Det): 0.1-1 μg/mL
IP: 2-8 μg/mg of lysate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3D Antibody Target Details CD3D Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
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