anti-Frog CD3E antibody for Immunoprecipitation

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Cryptomys amatus (Mole rat), Dog (Canine), Elephant, Frog, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
553
488
101
81
32
27
14
13
13
9
8
8
6
6
6
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
438
264
190
63
50
13
6
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
89
79
76
57
22
20
17
16
16
15
11
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
777
378
299
249
203
125
112
100
87
42
33
22
18
14
14
9
8
6
4
4
2
2
2
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN3026772
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN3026773 FACS IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6252412 FACS IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6296157 FACS IP WB Rabbit Log in to see Polyclonal 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Cryptomys amatus (Mole rat), Dog (Canine), Elephant, Frog, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
(553), (488), (101), (81), (32), (27), (14), (13), (13), (9), (8), (8), (6), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(438), (264), (190), (63), (50), (13), (6), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(89), (79), (76), (57), (22), (20), (17), (16), (16), (15), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(777), (378), (299), (249), (203), (125), (112), (100), (87), (42), (33), (22), (18), (14), (14), (9), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Characteristics This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Purification Protein A purified CD3e antibody
Immunogen Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
Isotype IgG
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Gene ID 916
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.\. FACS: use the CD3e antibody at 0.5-1 μg/million cells,WB: 0.5-1.0 μg/mL,IP: 1-2 μg/500 μg protein lysate,
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN3026772) Western blot testing of human Jurkat lysate in the absence and presence of immunizing...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN3026772) Western blot testing of Jurkat lysate with CD3e antibody.