anti-Guinea Pig CD3E antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 156-168, Cytoplasmic Domain
54
50
26
24
23
21
16
15
15
13
11
11
9
7
6
6
5
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
628
503
125
94
51
34
33
32
32
30
28
24
10
7
7
6
6
3
3
3
2
2
2
1
1
1
Host
Mouse
558
264
204
65
30
15
6
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
97
85
85
64
30
26
21
20
17
17
16
16
14
14
14
14
12
12
12
12
10
10
9
8
8
7
7
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
857
417
315
246
228
142
130
108
101
42
31
31
21
19
17
12
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6252415
$ 633.88
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4949515 FACS IF IHC (p) WB Mouse IgG1 AA 156-168, Cytoplasmic Domain Log in to see PC3-188A 0
1 ABIN4949516 FACS IF IHC (p) WB Mouse IgG1 AA 156-168, Cytoplasmic Domain Log in to see PC3-188A 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 156-168, Cytoplasmic Domain
(54), (50), (26), (24), (23), (21), (16), (15), (15), (13), (11), (11), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(628), (503), (125), (94), (51), (34), (33), (32), (32), (30), (28), (24), (10), (7), (7), (6), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(558), (264), (204), (65), (30), (15), (6), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(97), (85), (85), (64), (30), (26), (21), (20), (17), (17), (16), (16), (14), (14), (14), (14), (12), (12), (12), (12), (10), (10), (9), (8), (8), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(857), (417), (315), (246), (228), (142), (130), (108), (101), (42), (31), (31), (21), (19), (17), (12), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Clone PC3-188A
Isotype IgG1
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Gene ID 916
UniProt P07766
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes WB: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Optimal dilution of the CD3e antibody should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot and Store at -20C. Avoid freez-thaw cycles.