anti-Human CD3E antibody for ELISA

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Human
561
468
102
78
32
28
15
14
14
10
9
9
7
7
7
6
4
4
4
4
2
2
1
1
1
1
Host
Mouse
453
257
192
63
29
13
6
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
89
79
76
58
24
20
17
15
14
14
11
10
9
9
9
9
9
9
8
8
8
7
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
771
371
290
247
218
124
108
104
86
42
33
32
22
14
14
9
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN969024
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.25679 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
20.149809 ABIN396257 ELISA WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1 0
19.25679 ABIN931331 FACS ELISA WB Mouse IgG1 Log in to see 4E2 0
17.5 ABIN6260619 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
17.149809 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207 Log in to see 4C1 0
13.25679 ABIN5539697 ELISA WB Goat Internal Region Log in to see Polyclonal 0
13.25679 ABIN3183770 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
13.25679 ABIN4901213 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
13.25679 ABIN5618008 ELISA WB Mouse IgG1 kappa AA 23-126 Log in to see AT3H3 0
13.25679 ABIN2136517 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2136510 IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2136509 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2136511 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2136516 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
11.149809 ABIN514134 ELISA WB Mouse IgG2a kappa AA 23-207 Log in to see 3E12 0
10 ABIN1845772 FACS ELISA WB Mouse IgG1 Log in to see 0
7 ABIN2869505 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
7 ABIN3030389 ELISA WB Rabbit Ig Fraction AA 154-182 Log in to see Polyclonal 0
7 ABIN5574414 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
7 ABIN1575417 FACS ELISA WB Mouse Log in to see 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(561), (468), (102), (78), (32), (28), (15), (14), (14), (10), (9), (9), (7), (7), (7), (6), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(453), (257), (192), (63), (29), (13), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(89), (79), (76), (58), (24), (20), (17), (15), (14), (14), (11), (10), (9), (9), (9), (9), (9), (9), (8), (8), (8), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(771), (371), (290), (247), (218), (124), (108), (104), (86), (42), (33), (32), (22), (14), (14), (9), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN969024) Images
Purification Ascites
Immunogen Purified recombinant fragment of CD3E expressed in E. Coli.
Clone 4-00E-02
Isotype IgG1
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E antibody (ABIN969024) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Molecular Weight 23 kDa
Gene ID 916
HGNC 916
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E antibody (ABIN969024) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E antibody (ABIN969024) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-CD3E antibody (ABIN969024)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Boskovic, Arnold, Stilion, Gordon, Sim, Rivera-Calzada, Wienke, Isacke, Martinez-Pomares, Llorca: "Structural model for the mannose receptor family uncovered by electron microscopy of Endo180 and the mannose receptor." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8780-7, 2006

Urano, Iiduka, Sugiyama, Akiyama, Uzawa, Matsumoto, Kawasaki, Tashiro: "Involvement of the mouse Prp19 gene in neuronal/astroglial cell fate decisions." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7498-514, 2006

Xu, Call, Wucherpfennig: "A membrane-proximal tetracysteine motif contributes to assembly of CD3deltaepsilon and CD3gammaepsilon dimers with the T cell receptor." in: The Journal of biological chemistry, Vol. 281, Issue 48, pp. 36977-84, 2006

Li: "Therapeutic biology: checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference." in: Journal of cellular physiology, Vol. 209, Issue 3, pp. 695-700, 2006

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN969024) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN969024) Figure 1: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and n...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN969024) Figure 2: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.