anti-Human CD3E antibody for ELISA

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 23-208
54
50
26
25
18
16
15
15
13
12
11
9
7
6
6
5
5
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
567
474
119
96
45
33
33
31
31
27
26
21
10
8
7
7
6
4
4
4
2
2
2
1
1
1
Host
Mouse
472
260
197
63
29
13
6
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
91
80
78
59
25
21
17
16
15
15
12
11
10
10
10
10
10
10
9
9
8
8
8
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
781
391
315
247
223
125
108
104
86
42
33
32
22
19
14
9
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN396257
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.167359 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 4C1 0
12.167359 ABIN514134 ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 3E12 0
1 ABIN2869505 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
1 ABIN969024 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-02 5
1 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN6260619 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN5539697 ELISA WB Goat Cytosolic, Internal Region Log in to see Polyclonal 0
1 ABIN5629895 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
1 ABIN5629894 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 549 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
1 ABIN1997881 ELISA Mouse IgG1 AA 1-126, Extracellular Domain Log in to see 1A7E5G5 1
1 ABIN1977486 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN1845772 FACS ELISA WB Mouse IgG1 Log in to see 0
1 ABIN3183770 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN5959151 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4901213 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
1 ABIN3030389 ELISA WB Rabbit Ig Fraction AA 154-182 Log in to see Polyclonal 0
1 ABIN1453092 IHC ELISA WB Rabbit IgG AA 22-71 Log in to see Polyclonal 0
1 ABIN1955706 ELISA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1958047 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN467153 ELISA FACS Mouse IgG1 Log in to see 400 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 23-208
(54), (50), (26), (25), (18), (16), (15), (15), (13), (12), (11), (9), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(567), (474), (119), (96), (45), (33), (33), (31), (31), (27), (26), (21), (10), (8), (7), (7), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1)
Host Mouse
(472), (260), (197), (63), (29), (13), (6), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(91), (80), (78), (59), (25), (21), (17), (16), (15), (15), (12), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(781), (391), (315), (247), (223), (125), (108), (104), (86), (42), (33), (32), (22), (19), (14), (9), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: CD3E (AAH49847.1, 23 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 4C1
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background

Other Names: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E

Gene Description: CD3e molecule, epsilon (CD3-TCR complex)

Gene Summary: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq

Molecular Weight 23147 DA
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody (ABIN396257) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody (ABIN396257) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody (ABIN396257) CD3E monoclonal antibody (M04), clone 4C1 Western Blot analysis of CD3E expression in...
ELISA image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody (ABIN396257) Detection limit for recombinant GST tagged CD3E is approximately 1ng/ml as a capture ...
 image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-208) antibody (ABIN396257) Proximity Ligation Analysis of protein-protein interactions between CD247 and CD3E. H...