anti-Human CD3E antibody for Flow Cytometry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 1-126
29
27
23
20
16
15
15
14
12
10
9
7
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
517
493
123
89
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Mouse
473
232
204
65
22
15
5
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to PE
96
79
77
58
26
22
21
20
18
17
17
16
14
14
14
14
12
12
12
12
10
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
744
349
234
191
147
142
113
104
99
42
30
25
13
10
9
9
7
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN2213320
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.941537 ABIN94062 FACS Func IP PE Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
12.941537 ABIN94063 FACS Func IP APC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
12.941537 ABIN94060 FACS Func IP Biotin Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
12.941537 ABIN94061 FACS Func IP FITC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
12.941537 ABIN302049 FACS Func IP PerCP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 17
12.941537 ABIN638428 Func FACS IP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 12
12.941537 ABIN638434 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 12
12.941537 ABIN489928 FACS Func IHC (f) IP APC Mouse IgG1 Log in to see UCHT1 11
12.941537 ABIN302021 FACS Func IHC (f) IP FITC Mouse IgG1 Log in to see UCHT1 10
12.941537 ABIN489929 FACS Func IHC (f) IP PerCP Mouse IgG1 Log in to see UCHT1 9
12.941537 ABIN775704 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 9
12.941537 ABIN302043 FACS Func IHC (f) IP PE Mouse IgG1 Log in to see UCHT1 10
12.941537 ABIN4900707 CyTOF FACS ICC IP Mouse IgG1 Log in to see UCHT1 34
12.941537 ABIN4900708 CyTOF FACS ICC IP Mouse IgG1 Log in to see UCHT1 34
12.941537 ABIN4898324 FACS APC Mouse IgG1 Log in to see UCHT1 5
12.941537 ABIN4898333 FACS PE Mouse IgG1 Log in to see UCHT1 1
12.941537 ABIN4898323 FACS APC Mouse IgG1 Log in to see UCHT1 5
12.941537 ABIN4898327 FACS Fluorescein Mouse IgG1 Log in to see UCHT1 6
12.941537 ABIN4898328 FACS Fluorescein Mouse IgG1 Log in to see UCHT1 6
12.941537 ABIN4898334 FACS PE Mouse IgG1 Log in to see UCHT1 1

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 1-126
(29), (27), (23), (20), (16), (15), (15), (14), (12), (10), (9), (7), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(517), (493), (123), (89), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Mouse
(473), (232), (204), (65), (22), (15), (5), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to PE
(96), (79), (77), (58), (26), (22), (21), (20), (18), (17), (17), (16), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(744), (349), (234), (191), (147), (142), (113), (104), (99), (42), (30), (25), (13), (10), (9), (9), (7), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Specificity Human CD3e / CD3 epsilon
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon, ABIN2003682 NP_000724.1, Met1-Asp126, and conjugated with PE under optimum conditions, the unreacted PE was removed.
Immunogen Recombinant Human CD3e / CD3 epsilon protein (ABIN2003682)
Clone 001
Isotype IgG2a

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2213320) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE)
'Independent Validation' Badge
Lot Number HG08FE2109
Method validated Flow Cytometry
Positive Control peripheral blood mononuclear cells
Negative Control peripheral blood mononuclear cells
Notes This antibody performed well and is comparable to anti-CD3-FITC from BD Pharmingen (see data below) in terms of detecting a similar percentage of cells. The recommended amount of reagent to use per test is 1ul. You can see that even with using a small amount that the stained cells are very bright and are almost off scale. Even the negative peak has some spread to it. This antibody will need to be diluted further to obtain optimum staining.
Primary Antibody
  • Antigen: human CD3 (CD3e)
  • Catalog number: ABIN2104932
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: HG08FE2109
  • Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA
Controls
  • Positive control: peripheral blood mononuclear cells stained with anti-CD3
  • Negative control: peripheral blood mononuclear cells that are CD3 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protocol
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 1ul of anti-CD3-PE (Sino Biological) or 20ul of anti-CD3-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validation Images
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC (red),...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC from B...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...