anti-Human CD3E antibody for Flow Cytometry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 1-126
55
50
26
24
24
21
16
15
15
13
11
10
9
7
6
6
5
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
628
504
126
95
51
35
33
33
33
30
29
24
10
7
7
6
6
3
3
3
2
2
2
1
1
1
Host
Mouse
558
264
204
65
30
15
6
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to PE
97
85
84
64
30
26
21
20
17
17
16
16
14
14
14
14
12
12
12
12
10
10
9
8
8
7
7
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
857
418
315
247
228
143
130
108
101
42
31
31
21
19
17
12
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN2213320
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.728527 ABIN94063 FACS Func IP APC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
13.728527 ABIN94062 FACS Func IP PE Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
13.728527 ABIN94061 FACS Func IP FITC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
13.728527 ABIN94060 FACS Func IP Biotin Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
13.728527 ABIN302049 FACS Func IP PerCP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 17
13.728527 ABIN638428 Func FACS IP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 12
13.728527 ABIN638434 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 12
13.728527 ABIN4900707 CyTOF FACS ICC IP Mouse IgG1 Log in to see UCHT1 34
13.728527 ABIN489928 FACS Func IHC (f) IP APC Mouse IgG1 Log in to see UCHT1 11
13.728527 ABIN302021 FACS Func IHC (f) IP FITC Mouse IgG1 Log in to see UCHT1 10
13.728527 ABIN489929 FACS Func IHC (f) IP PerCP Mouse IgG1 Log in to see UCHT1 9
13.728527 ABIN775704 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 9
13.728527 ABIN302043 FACS Func IHC (f) IP PE Mouse IgG1 Log in to see UCHT1 10
13.728527 ABIN457363 FACS ICC IHC (f) IP WB PE Mouse IgG1 Activation Site Log in to see APA1-1 7
13.728527 ABIN4898328 FACS Fluorescein Mouse IgG1 Log in to see UCHT1 6
13.728527 ABIN4898324 FACS APC Mouse IgG1 Log in to see UCHT1 5
13.728527 ABIN969024 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-02 5
13.728527 ABIN2661400 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
13.728527 ABIN2662638 FACS PE Mouse IgG1 kappa Log in to see APA1-1 10
13.728527 ABIN2664657 FACS Mouse IgG1 kappa Log in to see APA1-1 10

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 1-126
(55), (50), (26), (24), (24), (21), (16), (15), (15), (13), (11), (10), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(628), (504), (126), (95), (51), (35), (33), (33), (33), (30), (29), (24), (10), (7), (7), (6), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(558), (264), (204), (65), (30), (15), (6), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to PE
(97), (85), (84), (64), (30), (26), (21), (20), (17), (17), (16), (16), (14), (14), (14), (14), (12), (12), (12), (12), (10), (10), (9), (8), (8), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(857), (418), (315), (247), (228), (143), (130), (108), (101), (42), (31), (31), (21), (19), (17), (12), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Specificity Human CD3e / CD3 epsilon
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon, ABIN2003682 NP_000724.1, Met1-Asp126, and conjugated with PE under optimum conditions, the unreacted PE was removed.
Immunogen Recombinant Human CD3e / CD3 epsilon protein (ABIN2003682)
Clone 001
Isotype IgG2a
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2213320) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE)
'Independent Validation' Badge
Lot Number HG08FE2109
Method validated Flow Cytometry
Positive Control peripheral blood mononuclear cells
Negative Control peripheral blood mononuclear cells
Notes This antibody performed well and is comparable to anti-CD3-FITC from BD Pharmingen (see data below) in terms of detecting a similar percentage of cells. The recommended amount of reagent to use per test is 1ul. You can see that even with using a small amount that the stained cells are very bright and are almost off scale. Even the negative peak has some spread to it. This antibody will need to be diluted further to obtain optimum staining.
Primary Antibody
  • Antigen: human CD3 (CD3e)
  • Catalog number: ABIN2104932
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: HG08FE2109
  • Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA
Controls
  • Positive control: peripheral blood mononuclear cells stained with anti-CD3
  • Negative control: peripheral blood mononuclear cells that are CD3 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protocol
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 1ul of anti-CD3-PE (Sino Biological) or 20ul of anti-CD3-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validation Images
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC (red),...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC from B...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 1-126) antibody (PE) (ABIN2104932) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...