anti-Human CD3E antibody for Immunocytochemistry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Human
553
468
102
82
32
28
15
14
14
10
9
9
7
7
7
4
4
4
4
2
2
1
1
1
1
Host
Mouse
437
265
190
63
29
13
6
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
78
74
55
22
20
17
15
14
14
11
10
9
9
9
9
9
9
8
8
8
7
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
756
377
297
247
201
125
112
99
87
42
32
22
18
14
13
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN4900707
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 6
20.897793 ABIN4900883 FACS ICC Mouse IgG1 Log in to see UCHT1R 0
19 ABIN3030388 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
17.897793 ABIN4898340 FACS ICC Alexa Fluor 594 Mouse IgG1 Log in to see UCHT1 0
17.5 ABIN6260619 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
4 ABIN1858299 ICC IHC WB Rabbit IgG AA 28-185 Log in to see Polyclonal 0
1 ABIN2618523 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
1 ABIN211097 ICC FACS IP WB Mouse IgG1 Log in to see 0
1 ABIN322964 ICC Mouse IgG2a Log in to see 0
1 ABIN199887 Agon ICC FACS IP Mouse IgG1 Log in to see 0
1 ABIN1575402 ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
-7.0434093 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1 0
-7.0434093 ABIN5629894 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 549 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
-7.0434093 ABIN5629895 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
-8.543409 ABIN4291485 FACS Func ICC IF IP Mouse IgG1 Log in to see TR66 5
-10.043409 ABIN4291521 FACS ICC IF IP WB Rabbit Log in to see Polyclonal 0
-10.043409 ABIN4957819 FACS ICC IF IHC (p) WB Alexa Fluor 488 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
-10.043409 ABIN4957840 FACS ICC IF IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see PC3-188A 0
-10.043409 ABIN4957839 FACS ICC IF IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see PC3-188A 0
-10.043409 ABIN4957837 FACS ICC IF IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see PC3-188A 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(553), (468), (102), (82), (32), (28), (15), (14), (14), (10), (9), (9), (7), (7), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(437), (265), (190), (63), (29), (13), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(87), (78), (74), (55), (22), (20), (17), (15), (14), (14), (11), (10), (9), (9), (9), (9), (9), (9), (8), (8), (8), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)
(756), (377), (297), (247), (201), (125), (112), (99), (87), (42), (32), (22), (18), (14), (13), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 34 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN4900707) Images
Specificity Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York, Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York, Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Characteristics Clone UCHT1 was used by HLDA to establish CD designation
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Human thymocytes (1),
Clone UCHT1
Isotype IgG1
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E antibody (ABIN4900707) Images back to top
Antigen
Alternative Name CD3 epsilon (CD3E Antibody Abstract)
Background Alternative Names: CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
Gene ID 916
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E antibody (ABIN4900707) Images back to top
Application Notes Flow Cytometry 0.25 μg/10^6 cells
Immunoprecipitation
T Cell Stimulation
Immunocytochemistry 8-25 μg/mL
CyTOF-reported
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E antibody (ABIN4900707) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD3E antibody (ABIN4900707)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Moore, Cui, Warsi, Durafourt, Zorko, Owen, Antel, Bar-Or: "Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 2, pp. 761-72, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Huang, Lu, Wu, Ouyang, Chen: "T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 54, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Patsoukis, Bardhan, Chatterjee, Sari, Liu, Bell, Karoly, Freeman, Petkova, Seth, Li, Boussiotis: "PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation." in: Nature communications, Vol. 6, pp. 6692, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Zhu, Huang, Stålesen, Hansson, Li: "Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation." in: Journal of thrombosis and haemostasis : JTH, Vol. 12, Issue 7, pp. 1156-65, 2014 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).

Ruan, Thorn, Liu, Li, Yang, Zhang, Zhang: "The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma." in: International journal of oncology, Vol. 44, Issue 6, pp. 2103-10, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Sgarbanti, Marsili, Remoli, Stellacci, Mai, Rotili, Perrotti, Acchioni, Orsatti, Iraci, Ferrari, Borsetti, Hiscott, Battistini: "IκB kinase ε targets interferon regulatory factor 1 in activated T lymphocytes." in: Molecular and cellular biology, Vol. 34, Issue 6, pp. 1054-65, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Renand, Milpied, Rossignol, Bruneau, Lemonnier, Dussiot, Coulon, Hermine: "Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs." in: PLoS ONE, Vol. 8, Issue 12, pp. e85589, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Fan, Zhang, Huang, Liu, Guo, Zhang, Luo: "Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif." in: PLoS ONE, Vol. 9, Issue 9, pp. e108253, 2014 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Wang, Xu, Zhou, Lo, Lau, Nangle, Yang, Zhang, Schimmel: "Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases." in: The Journal of biological chemistry, Vol. 288, Issue 41, pp. 29223-8, 2013 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Jones, Stumhofer, Foster, Twohig, Hertzog, Topley, Williams, Hunter, Jenkins, Wang, Jones: "Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 409-19, 2011 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).

Ramgolam, Sha, Marcus, Choudhary, Troiani, Chopra, Markovic-Plese: "B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 7, pp. 4518-26, 2011 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Pennino, Eyerich, Scarponi, Carbone, Eyerich, Nasorri, Garcovich, Traidl-Hoffmann, Albanesi, Cavani: "IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4880-8, 2010 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Eikawa, Ohue, Kitaoka, Aji, Uenaka, Oka, Nakayama: "Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 11, pp. 6734-40, 2010 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Longo, Tripiciano, Fiorelli, Bellino, Scoglio, Collacchi, Alvarez, Francavilla, Arancio, Paniccia, Lazzarin, Tambussi, Din, Visintini, Narciso, Antinori, DOffizi, Giulianelli, Carta, Di Carlo et al.: "Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up. ..." in: Vaccine, Vol. 27, Issue 25-26, pp. 3306-12, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Witte, Gruetz, Volk, Looman, Asadullah, Sterry, Sabat, Wolk et al.: "Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these ..." in: Genes and immunity, Vol. 10, Issue 8, pp. 702-14, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Hirosako, Goto, Fujii, Tsumori, Hirata, Tsumura, Kamohara, Kohrogi: "Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells." in: Clinical and experimental immunology, Vol. 155, Issue 2, pp. 266-74, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Lundqvist, Yokoyama, Smith, Berg, Childs: "Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells." in: Blood, Vol. 113, Issue 24, pp. 6120-7, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Ni, Walcheck: "Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation." in: Clinical immunology (Orlando, Fla.), Vol. 133, Issue 2, pp. 257-64, 2009 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Tran, Thacker, Louie, Oliver, White, Endres, Urquhart, Chung, Fox: "Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2989-98, 2008 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Stebbings, Findlay, Edwards, Eastwood, Bird, North, Mistry, Dilger, Liefooghe, Cludts, Fox, Tarrant, Robinson, Meager, Dolman, Thorpe, Bristow, Wadhwa, Thorpe, Poole: ""Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 5, pp. 3325-31, 2007 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN4900707) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN4900707) CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human periphe...
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN4900707) Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blo...