anti-Human CD3E antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Human, Non-Human Primate
562
468
102
78
32
28
15
14
14
10
9
9
7
7
7
5
4
4
4
4
2
2
1
1
1
1
Host
Mouse
454
257
192
63
29
13
6
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to APC
89
79
76
57
24
20
17
15
14
14
11
10
9
9
9
9
9
9
8
8
8
7
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
771
372
291
247
217
124
109
104
86
42
32
32
22
14
14
9
8
8
6
4
3
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN489928
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.252602 ABIN638434 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 12
25.252602 ABIN489929 FACS Func IHC (f) IP PerCP Mouse IgG1 Log in to see UCHT1 9
25.252602 ABIN775704 FACS Func IHC (f) IP Mouse IgG1 Log in to see UCHT1 9
25.252602 ABIN302021 FACS Func IHC (f) IP FITC Mouse IgG1 Log in to see UCHT1 10
25.252602 ABIN302043 FACS Func IHC (f) IP PE Mouse IgG1 Log in to see UCHT1 10
25.252602 ABIN457363 FACS ICC IHC (f) IP WB PE Mouse IgG1 Activation Site Log in to see APA1-1 7
22.252602 ABIN457415 FACS ICC IHC (f) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human, Non-Human Primate
(562), (468), (102), (78), (32), (28), (15), (14), (14), (10), (9), (9), (7), (7), (7), (5), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(454), (257), (192), (63), (29), (13), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to APC
(89), (79), (76), (57), (24), (20), (17), (15), (14), (14), (11), (10), (9), (9), (9), (9), (9), (9), (8), (8), (8), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(771), (372), (291), (247), (217), (124), (109), (104), (86), (42), (32), (32), (22), (14), (14), (9), (8), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN489928) Images
Specificity The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
HLDA I, WS Code T 3
HLDA III, WS Code T 126
HLDA III, WS Code T 471
HLDA VI, WS Code T 6T-CD3.1
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen human thymocytes followed by Sezary T cells
Clone UCHT1
Isotype IgG1
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E antibody (ABIN489928) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Gene ID 916
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E antibody (ABIN489928) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E antibody (ABIN489928) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD3E antibody (ABIN489928)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Background publications

Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004

Product cited in:

Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006

Lin, Liu, Chen, Wang, Medeiros, Hsu: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004

Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998

Fisch, Malkovsky, Braakman, Sturm, Bolhuis, Prieve, Sosman, Lam, Sondel: "Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis." in: The Journal of experimental medicine, Vol. 171, Issue 5, pp. 1567-79, 1990

Garson, Beverley, Coakham, Harper: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." in: Nature, Vol. 298, Issue 5872, pp. 375-7, 1982

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN489928) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (APC) (ABIN489928) Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific ...
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (APC) (ABIN489928) Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) APC.