anti-Human CD3E antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 23-207
54
50
25
25
18
16
15
15
12
12
11
9
7
6
6
5
5
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
566
472
119
94
45
33
33
31
31
27
26
21
10
8
7
7
6
4
4
4
2
2
2
1
1
1
Host
Rabbit
473
258
197
63
29
13
6
1
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
91
80
78
59
25
21
17
16
15
15
12
11
10
10
10
10
10
10
9
9
8
8
8
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
781
390
315
247
223
124
109
103
85
42
33
32
22
19
14
9
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN3043805
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.760653 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
19 ABIN3030388 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
16 ABIN5649343 IHC (p) WB Mouse IgG1 Log in to see CL1497 0
13 ABIN1497222 IF IHC IHC (p) WB Mouse IgG2b Log in to see 3E10 0
11.116879 ABIN213673 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
10 ABIN5649342 IHC (p) WB Mouse IgG1 Log in to see CL1466 0
8.5 ABIN385171 IHC IHC (p) Mouse IgG1, kappa Log in to see F7-2-38 1
7 ABIN1490980 IHC IHC (p) Rabbit IgG AA 156-168 Log in to see Polyclonal 0
7 ABIN672921 IF (p) IHC (p) WB Rabbit IgG AA 132-170 Log in to see Polyclonal 0
7 ABIN1732858 IHC (p) Rabbit IgG AA 156-168 Log in to see Polyclonal 0
7 ABIN5574434 IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN5574426 FACS IF IHC (p) WB Mouse IgG2b kappa Log in to see C3e-1308 0
7 ABIN5574427 FACS IF IHC (p) WB Mouse IgG2b kappa Log in to see C3e-1308 0
4 ABIN1385274 IF (p) IHC (p) WB Rabbit IgG AA 75-125 Log in to see Polyclonal 0
4 ABIN1732859 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN385865 IHC IHC (p) Mouse IgG2a Log in to see PS1 0
4 ABIN5959151 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN5773989 IHC (p) Rabbit C-Term Log in to see N26-R 0
4 ABIN5574429 FACS IF IHC (p) WB Mouse IgG1 Log in to see PC3-188A 0
4 ABIN5574430 FACS IF IHC (p) WB Mouse IgG1 Log in to see PC3-188A 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 23-207
(54), (50), (25), (25), (18), (16), (15), (15), (12), (12), (11), (9), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(566), (472), (119), (94), (45), (33), (33), (31), (31), (27), (26), (21), (10), (8), (7), (7), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(473), (258), (197), (63), (29), (13), (6), (1), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(91), (80), (78), (59), (25), (21), (17), (16), (15), (15), (12), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(781), (390), (315), (247), (223), (124), (109), (103), (85), (42), (33), (32), (22), (19), (14), (9), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN3043805) Images
Purpose Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain
Purification Immunogen affinity purified.
Immunogen E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Isotype IgG
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E antibody (ABIN3043805) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Synonyms: CD3 epsilon antibody|CD3e antibody|CD3E antibody|CD3e antigen epsilon polypeptide (TiT3 complex) antibody|CD3E antigen epsilon polypeptide antibody|CD3E antigen, epsilon subunit antibody|CD3e molecule epsilon antibody|CD3e molecule, epsilon (CD3 TCR complex) antibody|CD3e molecule, epsilon (CD3-TCR complex) antibody|CD3E_HUMAN antibody|IMD18 antibody|T cell antigen receptor complex epsilon subunit of T3 antibody|T cell surface antigen T3/Leu 4 epsilon chain antibody|T cell surface glycoprotein CD3 epsilon chain antibody|T-cell surface antigen T3/Leu-4 epsilon chain antibody|T-cell surface glycoprotein CD3 epsilon chain antibody|T3E antibody|TCRE antibody
Gene ID 916
UniProt P07766
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E antibody (ABIN3043805) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E antibody (ABIN3043805) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD3E antibody (ABIN3043805)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Guo, Qian, Zhang: "The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis." in: BMC ophthalmology, Vol. 16, pp. 66, 2017

Chen, Liu, Xu, Yang, Zhang, Zhang: "Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 1063-1067, 2015

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, 2013

Li, Geng, Yin, Chen, Yang, Wu, Li, Du, Schmidt, Przybylski: "Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients." in: Journal of translational medicine, Vol. 8, pp. 47, 2010

Xie, Jia, He, Gao: "Morphological observation of tumor infiltrating immunocytes in human rectal cancer." in: World journal of gastroenterology, Vol. 12, Issue 11, pp. 1757-60, 2006

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, 2005

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN3043805) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-6.JPG IHC(F): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093-7.JPG IHC(F): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml WB : Re...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, All lanes: Anti CD3 Epsilon at 0.5ug/ml Lane 1:...
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, IHC(P): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 23-207) antibody (ABIN3043805) Anti-CD3 epsilon Picoband antibody, pb9093IHC(F): Rat Spleen Tissue