anti-Human CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
Middle Region
29
27
23
20
16
15
15
14
12
11
9
7
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
519
495
123
89
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Rabbit
475
232
206
65
22
15
5
1
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
96
80
77
58
26
22
21
20
18
17
17
17
14
14
14
14
12
12
12
12
10
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
747
350
234
192
147
143
113
105
99
42
30
25
13
10
9
9
7
6
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2787934
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.16301 ABIN2705793 WB Rabbit Center Log in to see Polyclonal 0
3.16301 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
3.16301 ABIN2801427 WB Rabbit Log in to see Polyclonal 0
3.16301 ABIN969024 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-02 5
3.16301 ABIN657454 WB Rabbit Ig Fraction AA 154-182, C-Term Log in to see Polyclonal 0
3.16301 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.16301 ABIN441172 WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.16301 ABIN1871626 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.16301 ABIN5629894 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 549 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
3.16301 ABIN5629895 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
3.16301 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 4C1 0
3.16301 ABIN1858299 ICC IHC WB Rabbit IgG AA 28-185 Log in to see Polyclonal 0
3.16301 ABIN2681698 WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.16301 ABIN4291564 IF IHC WB Mouse IgG2b Log in to see 3E10 0
3.16301 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.16301 ABIN4291532 IHC IP WB Rabbit Log in to see Polyclonal 0
3.16301 ABIN3030388 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
3.16301 ABIN3026772 FACS IP WB Rabbit IgG Log in to see Polyclonal 0
3.16301 ABIN2681702 IP WB Rabbit IgG AA 1-126 Log in to see 801 0
3.16301 ABIN396257 WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope Middle Region
(29), (27), (23), (20), (16), (15), (15), (14), (12), (11), (9), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(519), (495), (123), (89), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Rabbit
(475), (232), (206), (65), (22), (15), (5), (1), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(96), (80), (77), (58), (26), (22), (21), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(747), (350), (234), (192), (147), (143), (113), (105), (99), (42), (30), (25), (13), (10), (9), (9), (7), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974673
Sequence QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against CD3E. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CD3E

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Alias Symbols: FLJ18683, T3E, TCRE
Protein Interaction Partner: UBC, CSK, ZAP70, SNTA1, PTPN22, PIK3R2, PIK3R1, LAT, LCK, CD38, CD247, CD3G, TRAT1, NCK2, UNC119, SHC1, NCK1, SYK, CD3D, NCL, CD79B, TOP2B, CD3EAP, JAK3,
Protein Size: 207
Molecular Weight 23 kDa
Gene ID 916
NCBI Accession NM_000733, NP_000724
UniProt P07766
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 207 AA

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Middle Region) antibody (ABIN2787934) WB Suggested Anti-CD3E Antibody Titration: 0.125ug/ml
ELISA Titer: 1...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Middle Region) antibody (ABIN2787934) WB Suggested Anti-CD3E
Antibody Titration: 0.125 µg/mL ELISA Titer: 1:.12500 <...