anti-Human CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
Middle Region
55
49
27
25
24
21
16
15
15
13
11
11
11
7
6
6
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
634
511
128
101
52
37
35
35
34
31
31
25
11
8
8
7
6
4
4
4
2
2
2
1
1
1
Host
Rabbit
560
269
204
65
30
15
6
1
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
96
85
85
64
30
26
21
20
17
17
16
16
14
14
14
14
12
12
12
12
10
10
9
8
8
7
7
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
862
425
319
253
229
143
131
109
101
42
33
32
22
19
17
12
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2787934
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0797327 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal 0
3.0797327 ABIN4291553 IHC WB Mouse IgG1 Log in to see CL1497 0
3.0797327 ABIN4291552 IHC WB Mouse IgG1 Log in to see CL1466 0
3.0797327 ABIN2705793 WB Rabbit Center Log in to see Polyclonal 0
3.0797327 ABIN2801427 WB Rabbit Log in to see Polyclonal 0
3.0797327 ABIN2869505 ELISA FACS WB Mouse IgG1 Log in to see 4E2 0
3.0797327 ABIN396257 ELISA WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1 0
3.0797327 ABIN969024 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-02 5
3.0797327 ABIN657454 WB Rabbit Ig Fraction AA 154-182, C-Term Log in to see Polyclonal 0
3.0797327 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.0797327 ABIN5949702 FACS IHC IHC (p) IP WB Rabbit IgG AA 100-250 Log in to see SY0239 0
3.0797327 ABIN441172 WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.0797327 ABIN4291484 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
3.0797327 ABIN4291483 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
3.0797327 ABIN1871626 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0797327 ABIN3022492 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0797327 ABIN5629894 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 549 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
3.0797327 ABIN5629895 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Mouse IgG2b kappa AA 23-119 Log in to see C3e-1308 0
3.0797327 ABIN1854857 IF/ICC IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.0797327 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 4C1 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope Middle Region
(55), (49), (27), (25), (24), (21), (16), (15), (15), (13), (11), (11), (11), (7), (6), (6), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(634), (511), (128), (101), (52), (37), (35), (35), (34), (31), (31), (25), (11), (8), (8), (7), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(560), (269), (204), (65), (30), (15), (6), (1), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(96), (85), (85), (64), (30), (26), (21), (20), (17), (17), (16), (16), (14), (14), (14), (14), (12), (12), (12), (12), (10), (10), (9), (8), (8), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(862), (425), (319), (253), (229), (143), (131), (109), (101), (42), (33), (32), (22), (19), (17), (12), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974673
Sequence QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against CD3E. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CD3E
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Alias Symbols: FLJ18683, T3E, TCRE
Protein Interaction Partner: UBC, CSK, ZAP70, SNTA1, PTPN22, PIK3R2, PIK3R1, LAT, LCK, CD38, CD247, CD3G, TRAT1, NCK2, UNC119, SHC1, NCK1, SYK, CD3D, NCL, CD79B, TOP2B, CD3EAP, JAK3,
Protein Size: 207
Molecular Weight 23 kDa
Gene ID 916
NCBI Accession NM_000733, NP_000724
UniProt P07766
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 207 AA

Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Middle Region) antibody (ABIN2787934) WB Suggested Anti-CD3E Antibody Titration: 0.125ug/ml
ELISA Titer: 1...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (Middle Region) antibody (ABIN2787934) WB Suggested Anti-CD3E
Antibody Titration: 0.125 µg/mL ELISA Titer: 1:.12500 <...