anti-Mouse (Murine) CD3E antibody for Cytometry by Time of Flight

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Mouse (Murine)
568
473
119
96
45
33
33
31
31
27
26
21
10
8
7
7
6
4
4
4
2
2
2
1
1
1
Host
Hamster
473
260
196
63
29
13
6
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to Alexa Fluor 488
91
80
78
59
25
21
17
16
15
14
12
11
10
10
10
10
10
10
9
9
8
8
8
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF)
780
392
315
246
223
125
109
104
86
42
33
32
22
19
13
9
9
8
6
4
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2657416
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658968 CyTOF ELISA (Capture) ELISPOT (Capture) ICC IHC IP Neut PE-Cy5 Hamster IgG Log in to see 145-2C11 9
1 ABIN2658969 CyTOF ELISA (Capture) ELISPOT (Capture) ICC IHC IP Neut PE-Cy5 Hamster IgG Log in to see 145-2C11 9
1 ABIN440731 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see APA1-1 0
1 ABIN4291602 CyTOF FACS IP WB Rabbit IgG Log in to see 145-2C11 0
1 ABIN4291604 CyTOF FACS Rabbit IgG Log in to see 500A2 0
1 ABIN4291606 CyTOF FACS IP WB Mouse IgG1 Log in to see 145-2C11 0
1 ABIN4291534 CyTOF FACS ICC IF IP WB Rabbit Log in to see Polyclonal 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(568), (473), (119), (96), (45), (33), (33), (31), (31), (27), (26), (21), (10), (8), (7), (7), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1)
Host Hamster
(473), (260), (196), (63), (29), (13), (6), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to Alexa Fluor 488
(91), (80), (78), (59), (25), (21), (17), (16), (15), (14), (12), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF)
(780), (392), (315), (246), (223), (125), (109), (104), (86), (42), (33), (32), (22), (19), (13), (9), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN2657416) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Clone 145-2C11
Isotype IgG
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E antibody (ABIN2657416) Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E antibody (ABIN2657416) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E antibody (ABIN2657416) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD3E antibody (ABIN2657416)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Background publications

Kinnaert, Pradier, Bournonville, Habrant, Goldman, Van Geertruyden et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal ..." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, 1997

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E antibody (ABIN2657416) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Alexa Fluor 488) (ABIN2657416) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Alexa Fluor 488)