anti-Rabbit CD3E antibody for Immunofluorescence

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 156-168, Cytoplasmic Domain
53
50
25
24
18
15
15
15
13
12
11
9
7
5
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
553
488
101
81
32
28
14
13
13
9
9
9
7
7
7
4
4
4
4
2
2
1
1
1
1
Host
Mouse
437
265
190
63
50
13
6
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
89
79
76
57
22
20
17
16
16
15
11
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
777
378
299
248
204
124
112
100
87
42
33
22
18
14
14
9
8
6
4
4
2
2
2
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN4949515
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN4949516 FACS IF IHC (p) WB Mouse IgG1 AA 156-168, Cytoplasmic Domain Log in to see PC3-188A 0
1 ABIN6252415 FACS IF IHC (p) WB Mouse IgG1 AA 156-168, Cytoplasmic Domain Log in to see PC3-188A 0
1 ABIN6294325 FACS IF IHC WB Mouse AA 156-168, Cytoplasmic Domain Log in to see 0
-5.0396347 ABIN4291521 FACS ICC IF IP WB Rabbit Log in to see Polyclonal 1
-12.539635 ABIN4291534 CyTOF FACS ICC IF IP WB Rabbit Log in to see Polyclonal 0
-12.539635 ABIN5949695 FACS ICC IF IHC IHC (p) WB DyLight 594 Mouse IgG1 AA 156-168 Log in to see PC3-188A 0
-12.539635 ABIN5949696 FACS ICC IF IHC IHC (p) Janelia Fluor® 549 Mouse IgG1 AA 156-168 Log in to see PC3-188A 0
-12.539635 ABIN5949698 FACS ICC IF IHC IHC (p) Janelia Fluor® 646 Mouse IgG1 AA 156-168 Log in to see PC3-188A 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 156-168, Cytoplasmic Domain
(53), (50), (25), (24), (18), (15), (15), (15), (13), (12), (11), (9), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(553), (488), (101), (81), (32), (28), (14), (13), (13), (9), (9), (9), (7), (7), (7), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(437), (265), (190), (63), (50), (13), (6), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(89), (79), (76), (57), (22), (20), (17), (16), (16), (15), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(777), (378), (299), (248), (204), (124), (112), (100), (87), (42), (33), (22), (18), (14), (14), (9), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Clone PC3-188A
Isotype IgG1
Plasmids, Primers & others

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal dilution of the CD3e antibody should be determined by the researcher.\. WB: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 156-168), (Cytoplasmic Domain) antibody (ABIN4949515) IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER...
SDS-PAGE (SDS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) (AA 156-168), (Cytoplasmic Domain) antibody (ABIN4949515) SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation ...