anti-Rabbit CD3E antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3e molecule
  • CD3 antigen, epsilon polypeptide
  • cd3e
  • CD3E
  • Cd3e
Epitope
AA 156-168, Cytoplasmic Domain
30
26
23
20
16
15
15
14
11
11
9
7
6
6
6
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
520
490
121
87
53
33
28
28
27
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Mouse
473
233
204
64
22
15
5
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
96
79
77
57
26
22
21
20
18
17
17
17
14
14
14
14
12
12
12
12
10
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
740
352
246
204
152
146
113
105
99
42
30
25
13
10
9
9
7
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4949515
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4949516 FACS IF IHC (p) WB Mouse IgG1 AA 156-168, Cytoplasmic Domain Log in to see PC3-188A 0

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Epitope AA 156-168, Cytoplasmic Domain
(30), (26), (23), (20), (16), (15), (15), (14), (11), (11), (9), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(520), (490), (121), (87), (53), (33), (28), (28), (27), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Mouse
(473), (233), (204), (64), (22), (15), (5), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(96), (79), (77), (57), (26), (22), (21), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(740), (352), (246), (204), (152), (146), (113), (105), (99), (42), (30), (25), (13), (10), (9), (9), (7), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Clone PC3-188A
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal dilution of the CD3e antibody should be determined by the researcher.\. WB: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).