anti-Cat (Feline) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Cat (Feline)
1270
579
228
69
61
40
32
30
28
26
25
25
22
21
18
18
15
12
10
10
10
9
7
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1321
517
313
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
248
216
152
127
41
40
38
32
30
29
28
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1644
540
471
301
285
270
215
165
91
73
49
39
31
26
21
19
18
14
14
11
11
9
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN135500
$ 360.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.463114 ABIN135503 FACS PE Mouse IgG1 Log in to see 3-4F4 2
18.463114 ABIN135501 FACS FITC Mouse IgG1 Log in to see 3-4F4 2
18.463114 ABIN135502 FACS Biotin Mouse IgG1 Log in to see 3-4F4 1
12.463114 ABIN288964 FACS IHC FITC Mouse IgG1 Log in to see 3-4F4 0
12.463114 ABIN288965 FACS IHC PE Mouse IgG1 Log in to see 3-4F4 0
12.463114 ABIN288963 FACS IP IHC Biotin Mouse IgG1 Log in to see 3-4F4 0
12.463114 ABIN284272 FACS IP IHC Mouse IgG1 Log in to see 3-4F4 0
9.856416 ABIN371323 FACS FITC Mouse IgG1 Log in to see 3-4F4 3
5.5 ABIN2480173 FACS FITC Mouse IgG1 Log in to see vpg34 3
1 ABIN371322 FACS Biotin Mouse IgG1 Log in to see 3-4F4 3
1 ABIN371325 FACS PE Mouse IgG1 Log in to see 3-4F4 3
1 ABIN2593257 FACS PE Mouse IgG1 Log in to see 3-4F4 0
1 ABIN226302 FACS IHC IHC (fro) IP Biotin Mouse IgG1, kappa Log in to see 0
1 ABIN371324 FACS IHC (fro) IP Mouse IgG1 Log in to see 3-4F4 3
1 ABIN2593256 FACS IHC IHC (fro) FITC Mouse IgG1 Log in to see 3-4F4 0
1 ABIN2593258 FACS IHC IHC (fro) IP Biotin Mouse IgG1 Log in to see 3-4F4 0
1 ABIN2593255 FACS IHC IHC (fro) IP Mouse IgG1 Log in to see 3-4F4 0
1 ABIN4900716 CyTOF FACS ICC WB Goat IgG Log in to see Polyclonal 0
1 ABIN182096 FACS FITC Mouse IgG1 Log in to see vpg34 0
1 ABIN641722 FACS FITC Mouse Log in to see 0

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Cat (Feline)
(1270), (579), (228), (69), (61), (40), (32), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (12), (10), (10), (10), (9), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1321), (517), (313), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(248), (216), (152), (127), (41), (40), (38), (32), (30), (29), (28), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(1644), (540), (471), (301), (285), (270), (215), (165), (91), (73), (49), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN135500) Images
Specificity Feline/Lion CD4, Mr 65 kDa
Characteristics Mouse Anti-Feline CD4-UNLB
Purification Purified
Immunogen Feline T cells
Clone 3-4F4
Isotype IgG1
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN135500) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55 % of thymocytes, 15 % of splenocytes, 40 % of lymph node cells, and 25 % of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN135500) Images back to top
Application Notes
  • Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Block - Reported in literature
  • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume 1 mL
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN135500) Images back to top
Concentration 0.5 mg/mL
Buffer 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
Preservative Without preservative
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C

References for anti-CD4 antibody (ABIN135500)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Webb, Quimby, Dow: "In vitro comparison of feline bone marrow-derived and adipose tissue-derived mesenchymal stem cells." in: Journal of feline medicine and surgery, Vol. 14, Issue 2, pp. 165-8, 2012

Katrin Helfer-Hungerbuehler, Cattori, Bachler, Hartnack, Riond, Ossent, Lutz, Hofmann-Lehmann: "Quantification and molecular characterization of the feline leukemia virus A receptor." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2011

Background publications

Dean, Quackenbush, Ackley, Cooper, Hoover: "Flow cytometric analysis of T-lymphocyte subsets in cats." in: Veterinary immunology and immunopathology, Vol. 28, Issue 3-4, pp. 327-35, 1991

Ackley, Hoover, Cooper: "Identification of a CD4 homologue in the cat." in: Tissue antigens, Vol. 35, Issue 2, pp. 92-8, 1990

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN135500) back to top
Supplier Images
 image for anti-CD4 Molecule (CD4) antibody (ABIN135500) anti-CD4 Molecule (CD4) antibody
ELISA image for anti-CD4 Molecule (CD4) antibody (ABIN135500) Feline peripheral blood lymphocytes were stained with Mouse Anti-Feline CD4-PE.