anti-Rat (Rattus) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Reactivity
Rat (Rattus)
1270
579
227
69
61
41
32
30
28
26
25
25
22
21
18
18
15
12
10
10
10
9
7
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1321
517
313
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to PE
248
215
152
127
41
40
38
32
30
29
28
25
24
20
16
15
15
15
15
15
15
14
14
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)
1644
539
471
301
285
270
215
165
90
73
49
39
31
26
21
19
18
14
14
11
11
9
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN2749065
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.17218 ABIN133174 FACS Mouse IgG1 Log in to see W3-25 0
21.405727 ABIN1302491 FACS ICC IHC Mouse IgG2a Log in to see OX-35 8
21.405727 ABIN2749063 ICC FACS IHC APC Mouse IgG2a Log in to see OX-35 9
21.405727 ABIN2749064 ICC FACS IHC FITC Mouse IgG2a Log in to see OX-35 9
14.266454 ABIN2480218 FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 4
12.766454 ABIN4900071 CyTOF FACS WB Sheep IgG AA 28-394 Log in to see Polyclonal 0
12.405727 ABIN133178 FACS FITC Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133179 FACS FITC Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133175 FACS Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133176 FACS Biotin Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133177 FACS Biotin Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133182 FACS PE Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN133181 FACS PE Mouse IgG1 Log in to see W3-25 0
12.405727 ABIN2192098 IHC (fro) FACS Mouse Log in to see 15-8A2 0
10 ABIN2689228 BR IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 9
10 ABIN2689241 FACS Biotin Mouse IgG2a, kappa Log in to see OX 7
10 ABIN2689232 IHC (f) IHC (fro) FACS Mouse IgG2a, kappa Log in to see OX 6
10 ABIN2689233 IHC (f) IHC (fro) FACS Mouse IgG2a, kappa Log in to see OX 7
10 ABIN2689227 IHC (fro) FACS IP Mouse IgG2a, kappa Log in to see OX 8
9.766454 ABIN120378 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Rat (Rattus)
(1270), (579), (227), (69), (61), (41), (32), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (12), (10), (10), (10), (9), (7), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1321), (517), (313), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to PE
(248), (215), (152), (127), (41), (40), (38), (32), (30), (29), (28), (25), (24), (20), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(1644), (539), (471), (301), (285), (270), (215), (165), (90), (73), (49), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN2749065) Images
Specificity The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
Cross-Reactivity (Details) Rat
Immunogen MLR generated rat Th cells
Clone OX-35
Isotype IgG2a
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN2749065) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN2749065) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN2749065) Images back to top
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD4 antibody (ABIN2749065)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Product cited in:

Viel, Lemarié, Benkirane, Paradis, Schiffrin: "Immune regulation and vascular inflammation in genetic hypertension." in: American journal of physiology. Heart and circulatory physiology, Vol. 298, Issue 3, pp. H938-44, 2010

Monzon-Casanova, Steiniger, Schweigle, Clemen, Zdzieblo, Starick, Müller, Wang, Rhost, Cardell, Pyz, Herrmann: "CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation." in: PLoS ONE, Vol. 5, Issue 9, 2010

Baba, Iwasaki, Maruoka, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara, Ishizu: "Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 2999-3006, 2008

Ramiro-Puig, Pérez-Cano, Ramos-Romero, Pérez-Berezo, Castellote, Permanyer, Franch, Izquierdo-Pulido, Castell: "Intestinal immune system of young rats influenced by cocoa-enriched diet." in: The Journal of nutritional biochemistry, Vol. 19, Issue 8, pp. 555-65, 2008

Baba, Ishizu, Iwasaki, Suzuki, Tomaru, Ikeda, Yoshiki, Kasahara: "CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype." in: Blood, Vol. 107, Issue 5, pp. 2004-12, 2006

Gelderman, Hultqvist, Holmberg, Olofsson, Holmdahl: "T cell surface redox levels determine T cell reactivity and arthritis susceptibility." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 34, pp. 12831-6, 2006

Yan, Devos, Yu, Xia, Rutgeerts, Goebels, Segers, Lin, Vandeputte, Waer: "Pathogenesis of autoimmunity after xenogeneic thymus transplantation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5936-46, 2003

Salomon, Netzer, Wildbaum, Schif-Zuck, Maor, Karin: "Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 5, pp. 2685-93, 2002

Tsuji, Kawaguchi, Wada, Nagoya, Inobe, Yamashita, Ishii, Uede: "Adenovirus-mediated in vivo B7-1 gene transfer induces anti-tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma." in: Cancer gene therapy, Vol. 9, Issue 9, pp. 747-55, 2002

Dragun, Lukitsch, Tullius, Qun, Park, Schneider, Luft, Haller: "Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat." in: Kidney international, Vol. 54, Issue 6, pp. 2113-22, 1999