anti-Rat (Rattus) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Epitope
Domain 1
69
53
29
20
16
15
15
15
13
9
8
8
6
6
6
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1272
584
236
71
61
41
36
30
28
26
25
25
22
21
18
18
15
15
13
12
10
9
9
7
5
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
1347
525
303
13
4
2
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
253
223
158
133
44
41
39
32
32
32
29
25
24
21
16
16
16
15
15
15
15
15
15
13
10
10
9
8
8
8
8
7
7
6
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1658
545
462
298
287
275
217
164
94
72
54
39
31
26
21
19
18
14
14
11
11
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120378
$ 709.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.311756 ABIN2480218 FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 4
14.311756 ABIN4900071 CyTOF FACS WB Sheep IgG AA 28-394 Log in to see Polyclonal 0
14.311756 ABIN2660683 FACS APC-Cy7 Mouse IgG1 kappa Log in to see W3-25 7
14.311756 ABIN2657419 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see W3-25 7
14.311756 ABIN2480228 IHC (fro) FACS IP Mouse IgG2a Domain 1 Log in to see OX-38 4
14.311756 ABIN2661701 FACS IP IHC WB FITC Mouse IgG1 kappa Log in to see W3-25 6
14.311756 ABIN2663438 FACS PE Mouse IgG1 kappa Log in to see W3-25 6
14.311756 ABIN2659510 FACS PE-Cy7 Mouse IgG1 kappa Log in to see W3-25 6
14.311756 ABIN2657776 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see W3-25 6
14.311756 ABIN2658653 FACS APC Mouse IgG1 kappa Log in to see W3-25 6
14.311756 ABIN5075610 FACS IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see W3-25 0
14.311756 ABIN2663441 FACS PE Mouse IgG2a kappa Log in to see OX-35 2
14.311756 ABIN2657779 FACS Alexa Fluor 647 Mouse IgG2a kappa Log in to see OX-35 2
14.311756 ABIN2661706 FACS IHC FITC Mouse IgG2a kappa Log in to see OX-38 1
14.311756 ABIN133174 FACS Mouse IgG1 Log in to see W3-25 0
1 ABIN120377 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN120376 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN120383 FACS IHC (fro) IP Mouse IgG2a Domain 2 Log in to see OX-35 8
1 ABIN1302491 FACS ICC IHC Mouse IgG2a Extracellular Log in to see OX-35 8
1 ABIN120372 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain 1
(69), (53), (29), (20), (16), (15), (15), (15), (13), (9), (8), (8), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1272), (584), (236), (71), (61), (41), (36), (30), (28), (26), (25), (25), (22), (21), (18), (18), (15), (15), (13), (12), (10), (9), (9), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(1347), (525), (303), (13), (4), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(253), (223), (158), (133), (44), (41), (39), (32), (32), (32), (29), (25), (24), (21), (16), (16), (16), (15), (15), (15), (15), (15), (15), (13), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(1658), (545), (462), (298), (287), (275), (217), (164), (94), (72), (54), (39), (31), (26), (21), (19), (18), (14), (14), (11), (11), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120378) Images
Purification Affinity chromatography on Protein A
Immunogen Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Clone W3-25
Isotype IgG1
Plasmids, Primers & others

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN120378) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN120378) Images back to top
Application Notes Flow cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN120378) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 antibody (ABIN120378)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Hofmann, Lachnit, Streppel, Witter, Neiss, Guntinas-Lichius, Angelov: "Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats." in: BMC immunology, Vol. 3, pp. 11, 2003

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Pelegrí, Franch, Castellote, Castell: "Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis." in: The Journal of rheumatology, Vol. 22, Issue 1, pp. 124-32, 1995

Clark, Jefferies, Barclay, Gagnon, Williams: "Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1649-53, 1987

Cantrell, Robins, Baldwin: "Rat lymphocyte subsets: cellular requirements for the generation of T-cell growth factor." in: Cellular immunology, Vol. 70, Issue 2, pp. 367-72, 1982

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Mason, Pugh, Webb: "The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies." in: Immunology, Vol. 44, Issue 1, pp. 75-87, 1981

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980

Williams, Galfrè, Milstein: "Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes." in: Cell, Vol. 12, Issue 3, pp. 663-73, 1978

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN120378) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) (Domain 1) antibody (ABIN120378) Staining of stimulated rat spleen cells with MOUSE ANTI RAT CD4 (ABIN120378).