anti-Human CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Epitope
AA 22-198
85
22
19
15
14
14
13
12
11
9
9
9
7
6
5
5
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
599
506
127
92
44
26
19
13
13
13
12
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
764
468
198
1
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
151
144
92
85
46
36
36
33
27
24
23
23
23
23
22
20
19
19
18
15
15
13
11
11
10
9
7
7
7
5
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
1159
446
367
257
246
236
202
184
173
35
33
20
16
13
13
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899645
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.30997 ABIN4896428 FACS ICC Alexa Fluor 488 Mouse IgG2b AA 22-198 Log in to see 37006
25.30997 ABIN4896429 FACS ICC Alexa Fluor 488 Mouse IgG2b AA 22-198 Log in to see 37006
1 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 9
1 ABIN2657487 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN1077936 ICC IHC IP WB Rabbit IgG AA 22-198 Log in to see Polyclonal
1 ABIN440359 FACS ICC IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RFT-8
1 ABIN440358 FACS ICC IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see RFT-8
1 ABIN2657488 Func ICC FACS IF ICFC IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see RPA-T8 6
1 ABIN5630736 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 594 Rat IgG2a Log in to see YTS105-18
1 ABIN4295991 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4262066 FACS ICC IF IHC IHC (fro) Alexa Fluor 405 Rat IgG2a Log in to see YTS105-18
1 ABIN4262070 FACS ICC IF IHC IHC (fro) DyLight 350 Rat IgG2a Log in to see YTS105-18
1 ABIN4262069 FACS ICC IF IHC IHC (fro) Alexa Fluor 700 Rat IgG2a Log in to see YTS105-18
1 ABIN4262068 FACS ICC IF IHC IHC (fro) Alexa Fluor 647 Rat IgG2a Log in to see YTS105-18
1 ABIN4262074 FACS ICC IF IHC IHC (fro) DyLight 550 Rat IgG2a Log in to see YTS105-18
1 ABIN4262077 FACS ICC IF IHC IHC (fro) DyLight 755 Rat IgG2a Log in to see YTS105-18
1 ABIN4262073 FACS ICC IF IHC IHC (fro) DyLight 488 Rat IgG2a Log in to see YTS105-18
1 ABIN4262076 FACS ICC IF IHC IHC (fro) DyLight 680 Rat IgG2a Log in to see YTS105-18
1 ABIN4262067 FACS ICC IF IHC IHC (fro) Alexa Fluor 488 Rat IgG2a Log in to see YTS105-18
1 ABIN4262071 FACS ICC IF IHC IHC (fro) DyLight 405 Rat IgG2a Log in to see YTS105-18

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 22-198
(85), (22), (19), (15), (14), (14), (13), (12), (11), (9), (9), (9), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(599), (506), (127), (92), (44), (26), (19), (13), (13), (13), (12), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Mouse
(764), (468), (198), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(151), (144), (92), (85), (46), (36), (36), (33), (27), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (15), (13), (11), (11), (10), (9), (7), (7), (7), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
(1159), (446), (367), (257), (246), (236), (202), (184), (173), (35), (33), (20), (16), (13), (13), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4899645) Images
Specificity Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human CD8 alpha , Ser22-Val198, Accession # NP_741969
Clone 37006
Isotype IgG2b

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN4899645) Images back to top
Antigen
Alternative Name CD8 alpha (CD8A Antibody Abstract)
Gene ID 925
NCBI Accession NP_741969
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN4899645) Images back to top
Application Notes Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN4899645) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD8 antibody (ABIN4899645)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Chimote, Hajdu, Sfyris, Gleich, Wise-Draper, Casper, Conforti: "Kv1.3 Channels Mark Functionally Competent CD8+ Tumor-Infiltrating Lymphocytes in Head and Neck Cancer." in: Cancer research, Vol. 77, Issue 1, pp. 53-61, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4899645) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-CD8a Molecule (CD8A) (AA 22-198) antibody (ABIN4899645) anti-CD8a Molecule (CD8A) (AA 22-198) antibody