anti-Mouse (Murine) CD80 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Reactivity
Dog (Canine), Mouse (Murine)
458
291
48
11
9
6
5
3
2
2
1
1
Host
Armenian Hamster
412
120
100
99
40
19
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
76
75
56
41
20
20
18
16
12
10
10
8
8
8
8
7
7
7
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
533
212
148
143
121
88
72
42
38
29
26
18
9
7
7
6
6
5
5
4
2
2
2
2
1
1
1
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Catalog No. ABIN2689407
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN567985 Func IHC (fro) FACS IP Hamster IgG2 Log in to see 16-10A1 9
1 ABIN1176972 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16-10A1 6
1 ABIN489934 Func FACS IHC (fro) IP Hamster IgG2 Log in to see 16-10A1 8
1 ABIN316880 FACS IHC (fro) Rat IgG2a Log in to see RM80 2
1 ABIN475531 FACS IHC (fro) IP Hamster IgG Log in to see 16-10A1 0
1 ABIN316878 Func FACS IHC (fro) Rat IgG2a Log in to see RM80 2
1 ABIN2472610 IHC (fro) FACS Rat IgG2a Log in to see RM80 4
1 ABIN207222 FACS IHC (fro) FITC Rat IgG2a Log in to see RM80 0
1 ABIN576900 FACS IHC (fro) PE Rat IgG2a Log in to see RM80 0
1 ABIN600885 FACS IHC (fro) Rat IgG2a Log in to see 0
1 ABIN600887 FACS IHC (fro) Rat IgG2a Log in to see 0
1 ABIN206612 FACS IHC (fro) Rat IgG2a Log in to see RM80 0
1 ABIN3200026 FACS Func IHC (fro) IP Armenian Hamster IgG2 Log in to see 16-10A1 0
1 ABIN1967873 BR FACS Func IHC (fro) IP Neut Hamster IgG2 Log in to see 16-10A1 0
1 ABIN600886 FACS Func IHC (fro) Rat IgG2a Log in to see 0
1 ABIN4282666 FACS Func IHC IHC (fro) IP Armenian Hamster IgG Log in to see 16-10A1 0
1 ABIN4282667 FACS Func IHC IHC (fro) IP Armenian Hamster IgG Log in to see 16-10A1 0
1 ABIN4282668 FACS Func IHC IHC (fro) IP Armenian Hamster IgG Log in to see 16-10A1 0
1 ABIN4282669 FACS Func IHC IHC (fro) IP Armenian Hamster IgG Log in to see 16-10A1 0
1 ABIN4255280 FACS IHC IHC (fro) Alexa Fluor 405 Armenian Hamster IgG Log in to see 16-10A1 0

General

Antigen CD80 Antibodies
Reactivity Dog (Canine), Mouse (Murine)
(458), (291), (48), (11), (9), (6), (5), (3), (2), (2), (1), (1)
Host Armenian Hamster
(412), (120), (100), (99), (40), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(76), (75), (56), (41), (20), (20), (18), (16), (12), (10), (10), (8), (8), (8), (8), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(533), (212), (148), (143), (121), (88), (72), (42), (38), (29), (26), (18), (9), (7), (7), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN2689407) Images
Brand BD Pharmingen™
Characteristics The 16-10A1 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN-γ-activated alveolar macrophages of the dog. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Hamster Anti-Mouse CD80 - Purified - Clone 16-10A1 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms, Dog - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CD80 (B7) Transfected Cell Line
Clone 16
Isotype IgG2, kappa

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN2689407) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background Synonyms: B7-1
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN2689407) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN2689407) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD80 antibody (ABIN2689407)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, 1997

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Boussiotis, Gribben, Freeman, Nadler: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, 1995

Harlan, Hengartner, Huang, Kang, Abe, Moreadith, Pircher, Gray, Ohashi, Freeman: "Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 8, pp. 3137-41, 1994

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992