anti-Mouse (Murine) CD86 antibody for Intracellular Flow Cytometry

Recommended CD86 Antibody

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Mouse (Murine)
369
348
133
10
9
6
2
2
1
1
Host
Rat
304
238
127
Clonality
Monoclonal
Conjugate
This CD86 antibody is conjugated to PE-Cy7
89
85
46
41
14
10
9
9
8
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
481
220
163
137
90
82
71
55
41
28
19
13
11
8
6
3
2
2
1
1
1
Options

Available images

Catalog No. ABIN2659560
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN2659556 ICFC PE-Cy7 Rat IgG2a kappa GL-1 11
4 ABIN2659014 ICFC PE-Cy5 Rat IgG2a kappa GL-1 11
1 ABIN2659557 ICFC PE-Cy7 Rat IgG2a kappa GL-1 13
1 ABIN2659561 ICFC PE-Cy7 Rat IgG2b kappa PO3 12
1 ABIN2659015 ICFC PE-Cy5 Rat IgG2a kappa GL-1 11

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Mouse (Murine)
(369), (348), (133), (10), (9), (6), (2), (2), (1), (1)
Host Rat
(304), (238), (127)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is conjugated to PE-Cy7
(89), (85), (46), (41), (14), (10), (9), (9), (8), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(481), (220), (163), (137), (90), (82), (71), (55), (41), (28), (19), (13), (11), (8), (6), (3), (2), (2), (1), (1), (1)
Pubmed 12 references available
Supplier

Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN2659560) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Clone PO3
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN2659560) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN2659560) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN2659560) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD86 antibody (ABIN2659560)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Background publications

Bhatnagar, Schorey: "Exosomes released from infected macrophages contain Mycobacterium avium glycopeptidolipids and are proinflammatory." in: The Journal of biological chemistry, Vol. 282, Issue 35, pp. 25779-89, 2007

Giroux, Yurchenko, St-Pierre, Piccirillo, Perreault: "T regulatory cells control numbers of NK cells and CD8alpha+ immature dendritic cells in the lymph node paracortex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4492-502, 2007

Lawson, Manenkova, Ahamed, Chen, Zou, Baccala, Theofilopoulos, Yuan: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007

Ma, Xu, An, Dong, Lin, Shi, Wu: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006

Carreno, Collins: "The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses." in: Annual review of immunology, Vol. 20, pp. 29-53, 2002

Kasprowicz, Kohm, Berton, Chruscinski, Sharpe, Sanders: "Stimulation of the B cell receptor, CD86 (B7-2), and the beta 2-adrenergic receptor intrinsically modulates the level of IgG1 and IgE produced per B cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 680-90, 2000

Nakajima, Kodama, Morimoto, Azuma, Takeda, Oshima, Yoshino, Yagita, Okumura: "Antitumor effect of CD40 ligand: elicitation of local and systemic antitumor responses by IL-12 and B7." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 4, pp. 1901-7, 1998

Saito, Sakurai, Ohata, Kohsaka, Hashimoto, Okumura, Abe, Azuma: "Involvement of CD40 ligand-CD40 and CTLA4-B7 pathways in murine acute graft-versus-host disease induced by allogeneic T cells lacking CD28." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 9, pp. 4225-31, 1998

Nuriya, Yagita, Okumura, Azuma: "The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity." in: International immunology, Vol. 8, Issue 6, pp. 917-26, 1997

Nakajima, Azuma, Kodera, Nuriya, Terashi, Abe, Hirose, Shirai, Yagita, Okumura: "Preferential dependence of autoantibody production in murine lupus on CD86 costimulatory molecule." in: European journal of immunology, Vol. 25, Issue 11, pp. 3060-9, 1996

Freeman, Borriello, Hodes, Reiser, Hathcock, Laszlo, McKnight, Kim, Du, Lombard: "Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 907-9, 1993

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, 1993

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN2659560) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (PE-Cy7) (ABIN2659560) anti-CD86 Molecule (CD86) antibody (PE-Cy7)