anti-Mouse (Murine) CD86 antibody for Immunofluorescence

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Mouse (Murine)
461
413
181
10
6
3
2
2
1
1
Host
Rat
379
310
110
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
99
96
55
49
21
15
13
11
10
9
9
8
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
614
250
179
176
132
96
87
69
55
54
44
16
13
12
12
11
8
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2689420
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.313191 ABIN3197982 IC IF IP WB Rabbit Log in to see Polyclonal 0
10 ABIN6185458 FACS IF IHC (f) WB APC Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185465 FACS IF IHC (f) WB PerCP Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185466 FACS IF IHC (f) WB PE Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN6185469 FACS IF IHC (f) WB Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN3025928 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see C86-1146 0
10 ABIN3024504 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CDLA86 0
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Log in to see BU63 0
7 ABIN3026659 FACS IF IHC (p) Mouse IgG1, kappa Log in to see BU63 0
4 ABIN2661840 FACS IF FITC Rat IgG2b kappa Log in to see PO3 12
4 ABIN2661838 FACS IF FITC Rat IgG2a kappa Log in to see GL-1 11
4 ABIN3024502 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SPM600 0
1 ABIN2661839 FACS IF FITC Rat IgG2b kappa Log in to see PO3 12
1 ABIN2661837 FACS IF FITC Rat IgG2a kappa Log in to see GL-1 11
1 ABIN6185404 FACS IF IHC (f) IP WB CF®555 Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185410 FACS IF IHC (f) IP WB CF®770 Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185412 FACS IF IHC (f) IP WB CF®680 Mouse IgG1, kappa Log in to see BU63 0

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Mouse (Murine)
(461), (413), (181), (10), (6), (3), (2), (2), (1), (1)
Host Rat
(379), (310), (110), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(99), (96), (55), (49), (21), (15), (13), (11), (10), (9), (9), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
(614), (250), (179), (176), (132), (96), (87), (69), (55), (54), (44), (16), (13), (12), (12), (11), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN2689420) Images
Brand BD Pharmingen™
Characteristics The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture. In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity. CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4). GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells. In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Upregulation of membrane CD86 (B7-2) on activated B lymphocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and either unstained (shaded histograms) or stained with purified GL1 mAb (open histograms) followed by FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896, shaded and open histograms). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse CD86 - Purified - Clone GL1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse (CBA/Ca) LPS-activated splenic B Cells
Clone GL1
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN2689420) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background Synonyms: B7-2
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN2689420) Images back to top
Application Notes Flow Cytometry System. Resting lymphocytes (left panel) or activated blasts (right panel) were selected according to light-scatter profile.
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN2689420) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD86 antibody (ABIN2689420)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Turley, Inaba, Garrett, Ebersold, Unternaehrer, Steinman, Mellman: "Transport of peptide-MHC class II complexes in developing dendritic cells." in: Science (New York, N.Y.), Vol. 288, Issue 5465, pp. 522-7, 2000

Martín-Fontecha, Assarsson, Carbone, Kärre, Ljunggren: "Triggering of murine NK cells by CD40 and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5910-6, 1999

McAdam, Schweitzer, Sharpe: "The role of B7 co-stimulation in activation and differentiation of CD4+ and CD8+ T cells." in: Immunological reviews, Vol. 165, pp. 231-47, 1999

Hakamada-Taguchi, Kato, Ushijima, Murakami, Uede, Nariuchi: "Expression and co-stimulatory function of B7-2 on murine CD4+ T cells." in: European journal of immunology, Vol. 28, Issue 3, pp. 865-73, 1998

Borriello, Sethna, Boyd, Schweitzer, Tivol, Jacoby, Strom, Simpson, Freeman, Sharpe: "B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation." in: Immunity, Vol. 6, Issue 3, pp. 303-13, 1997

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, 1997

Liu, Wenger, Zhao, Nielsen: "Distinct costimulatory molecules are required for the induction of effector and memory cytotoxic T lymphocytes." in: The Journal of experimental medicine, Vol. 185, Issue 2, pp. 251-62, 1997

Nikcevich, Gordon, Tan, Hurst, Kroepfl, Gardinier, Barrett, Miller: "IFN-gamma-activated primary murine astrocytes express B7 costimulatory molecules and prime naive antigen-specific T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 614-21, 1997

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994

Inaba, Witmer-Pack, Inaba, Hathcock, Sakuta, Azuma, Yagita, Okumura, Linsley, Ikehara, Muramatsu, Hodes, Steinman: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, 1994

Larsen, Ritchie, Hendrix, Linsley, Hathcock, Hodes, Lowry, Pearson: "Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 11, pp. 5208-19, 1994

Lenschow, Su, Zuckerman, Nabavi, Jellis, Gray, Miller, Bluestone: "Expression and functional significance of an additional ligand for CTLA-4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11054-8, 1994

Freeman, Borriello, Hodes, Reiser, Hathcock, Laszlo, McKnight, Kim, Du, Lombard: "Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 907-9, 1993

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, 1993